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首页> 外文期刊>International Journal of Zoology >Noninvasive Method for a Statewide Survey ofEastern Hellbenders Cryptobranchus alleganiensis UsingEnvironmental DNA
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Noninvasive Method for a Statewide Survey ofEastern Hellbenders Cryptobranchus alleganiensis UsingEnvironmental DNA

机译：使用环境DNA对东部地狱弯腰隐花草进行全州调查的非侵入性方法

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摘要

Traditional survey methods of aquatic organisms may be difficult, lengthy, and destructive to the habitat. Some methods are invasive and can be harmful to the target species. The use of environmental DNA (eDNA) has proven to be effective at detectinglow population density aquatic macroorganisms. This study refined the technique to support statewide surveys. Hellbender presence was identified by using hellbender specific primers (cytochrome b gene) to detect eDNA in water samples collected at rivers,streams and creeks in Ohio and Kentucky with historical accounts of the imperiled eastern hellbender (Cryptobranchus a. alleganiensis). Two sampling protocols are described; both significantly reduced the amount of water required for collection from thepreviously described 6 L collection. Two-liter samples were adequate to detect hellbender presence in natural waterways where hellbenders have been previously surveyed in both Ohio and Kentucky—1L samples were not reliable. DNA extracted from 3 L of water collected onto multiple filters (1 L/filter) could be combined and concentrated through ethanol precipitation, supporting amplification of hellbender DNA and dramatically reducing the filtration time. This method improves the efficiency and welfare implications of sampling methods for reclusive aquatic species of low population density for statewide surveys that involve collecting from multiple watersheds.
机译：传统的水生生物调查方法可能难以，冗长且对栖息地造成破坏。一些方法是侵入性的，可能对目标物种有害。事实证明，使用环境DNA（eDNA）可有效检测低种群密度的水生生物。这项研究完善了该技术以支持全州范围的调查。通过使用hellbender特异性引物（cytochrome b基因）检测在俄亥俄州和肯塔基州的河流，溪流和小河中收集的水样中的eDNA，从而鉴定了hellbender的存在，该历史记载了东部的hellbender濒危（Cryptobranchus a。alleganiensis）。描述了两种采样协议;两者都大大减少了先前描述的6 L收集中收集所需的水量。两升的样品足以检测以前在俄亥俄州和肯塔基州均曾调查过地狱动物的自然水道中的地狱动物存在-1升样本并不可靠。从收集到多个过滤器（1升/过滤器）中的3升水中提取的DNA可以合并并通过乙醇沉淀进行浓缩，从而支持hellbender DNA的扩增并大大减少了过滤时间。这种方法提高了抽样方法的效率和福利意义，该方法适用于涉及从多个流域收集的全州范围调查的低人口密度隐居水生物种。

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