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首页> 外文期刊>Investigative ophthalmology & visual science >N-methyl-D-aspartate receptor subunit NR3A in the retina: developmental expression, cellular localization, and functional aspects.
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N-methyl-D-aspartate receptor subunit NR3A in the retina: developmental expression, cellular localization, and functional aspects.

机译：视网膜中的N-甲基-D-天冬氨酸受体亚基NR3A：发育表达，细胞定位和功能方面。

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PURPOSE. Recently, a novel N-methyl-D-aspartate receptor (NMDAR) subunit, NR3A, has been discovered in the brain and shown to decrease NMDAR activity by modulating the calcium permeability of the receptor channel. The insertion of NR3A within the NMDAR complex may thus alter NMDAR properties and play a crucial role during processes of neuronal development and degeneration. The present study is the first to investigate the expression and cellular localization of NR3A on the protein level in the retina and to elucidate its putative functional roles within the retinal circuitry. METHODS. The expression of NR3A in the retina was analyzed by reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blot analysis. Functional aspects of NR3A in the retina were addressed by measuring the NMDA-induced increase in intracellular calcium, [Ca(2+)](i), in retinal cells prepared from wild-type (NR3A(+/+)) and NR3A knockout (NR3A(+/-), and NR3A(-/-)) mice. RESULTS. NR3A protein expression was initially observed in the first postnatal week and was predominantly localized to cell bodies in the ganglion cell layer. In older animals, two bands of NR3A immunoreactivity were additionally observed in the inner plexiform layer. NMDA-evoked [Ca(2+)](i) responses were found to be significantly greater in retinal cells in NR3A(-/-) mice than in wild-type retinas. CONCLUSIONS. The data indicate that NR3A is specifically expressed in the inner retina and may modulate NMDAR-mediated calcium influx and thus [Ca(2+)](i) levels in retinal ganglion cells and amacrine cells.
机译：目的。最近，在大脑中发现了一种新型的N-甲基-D-天冬氨酸受体（NMDAR）亚基NR3A，并显示通过调节受体通道的钙渗透性降低NMDAR活性。因此，NR3A在NMDAR复合物中的插入可能会改变NMDAR的性质，并在神经元发育和变性过程中起关键作用。本研究是第一个研究NR3A在视网膜蛋白水平上的表达和细胞定位，并阐明其在视网膜回路中的假定功能。方法。 NR3A在视网膜中的表达通过逆转录聚合酶链反应（RT-PCR），免疫组织化学和蛋白质印迹分析进行分析。 NR3A在视网膜中的功能方面已通过测量由野生型（NR3A（+ / +））和NR3A敲除制备的视网膜细胞中NMDA诱导的细胞内钙[Ca（2 +）]（i）的增加来解决（NR3A（+/-）和NR3A（-/-））小鼠。结果。 NR3A蛋白表达最初是在产后第一周观察到的，并且主要定位在神经节细胞层的细胞体中。在年长的动物中，在内侧丛状层中还观察到两条NR3A免疫反应性带。发现NMDA诱发的[Ca（2 +）]（i）反应在NR3A（-/-）小鼠的视网膜细胞中比在野生型视网膜中明显更大。结论。数据表明NR3A在内部视网膜中特异性表达，并可能调节NMDAR介导的钙内流，从而调节视网膜神经节细胞和无长突细胞中的[Ca（2 +）]（i）水平。

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