...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biofabrication >Micropattern array with gradient size (PAGS) plastic surfaces fabricated by PDMS (polydimethylsiloxane) mold-based hot embossing technique for investigation of cell-surface interaction
【24h】

Micropattern array with gradient size (PAGS) plastic surfaces fabricated by PDMS (polydimethylsiloxane) mold-based hot embossing technique for investigation of cell-surface interaction

机译：通过基于PDMS（聚二甲基硅氧烷）模具的热压花技术制造的具有梯度尺寸（PAGS）塑料表面的微图案阵列，用于研究细胞表面相互作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Recently, it was found that the variations of physical environment significantly affect cell behaviors including cell proliferation, migration and differentiation. Through a plastic surface with controlled mechanical properties such as stiffness, one can change the orientation and migration of cells in a particular direction, thereby determining cell behaviors. In this study, we demonstrate a polydimethylsiloxane (PDMS) mold-based hot embossing technique for rapid, simple and low-cost replication of polystyrene (PS) surfaces having micropatterns. The PDMS mold was fabricated by UV-photolithography followed by PDMS casting; the elastomeric properties of PDMS enabled us to obtain conformal contact of the PDMS mold to a PS surface and to create high transcription quality of micropatterns on the PS surface. Two different types of circular micropillar and microwell arrays were successfully replicated on the PS surfaces based on the suggested technique. The micropatterns were designed to have various diameters (2-150m), spacings (2-160m) and heights (1.4, 2.4, 8.2 and 14.9m), so as to generate the gradient of physical properties on the surface. Experimental parametric studies indicated that (1) the embossing temperature became a critical processing parameter as the aspect ratio of micropattern increased and (2) the PDMS mold-based hot embossing could successfully replicate micropatterns, even having an aspect ratio of 2.7 for micropattern diameter of 6m, with an optimal processing condition (embossing pressure and temperature of 0.4 MPa and 130°C, respectively) in this study. We carried out cell experiments with adipose-derived stem cells on the replicated PS surface with the height of 1.4m to investigate cellular behaviors in response to the micropattern array with gradient size. Cellular experiment results showed that the micropillar-arrayed surface improved cell proliferation as compared with the microwell-arrayed surface. We could also estimate the ranges of pattern sizes having the desired effects on the cellular behaviors.
机译：最近，发现物理环境的变化显着影响细胞行为，包括细胞增殖，迁移和分化。通过具有受控机械特性（例如刚度）的塑料表面，可以改变细胞在特定方向上的定向和迁移，从而确定细胞行为。在这项研究中，我们演示了基于聚二甲基硅氧烷（PDMS）模具的热压花技术，可快速，简单且低成本地复制具有微图案的聚苯乙烯（PS）表面。 PDMS模具是通过UV光刻，随后进行PDMS浇铸来制造的; PDMS的弹性特性使我们能够将PDMS模具与PS表面进行共形接触，并在PS表面上创建高品质的微图案转录。基于建议的技术，两种不同类型的圆形微柱和微孔阵列已成功复制到PS表面上。将微图案设计为具有各种直径（2-150m），间距（2-160m）和高度（1.4、2.4、8.2和14.9m），以便在表面上生成物理特性的梯度。实验参数研究表明（1）随着微图案长宽比的增加，压花温度成为关键的加工参数;（2）基于PDMS模具的热压花可以成功复制微图案，即使微图案直径为2.7时长宽比也是如此。在本研究中，最佳加工条件为6m（压花压力和温度分别为0.4 MPa和130°C）。我们在复制的PS表面上以1.4m的高度对脂肪来源的干细胞进行了细胞实验，以研究对具有梯度大小的微模式阵列响应的细胞行为。细胞实验结果表明，与微孔排列的表面相比，微柱排列的表面改善了细胞增殖。我们还可以估计对细胞行为具有所需影响的图案大小范围。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号