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Raising Environmental Awareness Through Applied Biochemistry Laboratory Experiments

机译：通过应用生物化学实验室实验提高环境意识

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摘要

Our environment is under constant pressure and threat from various sources of pollution. Science students, in particular chemistry students, must not only be made aware of these issues, but also be taught that chemistry (and science) can provide solutions to such real-life issues. To this end, a newly developed biochemistry laboratory experiment is described that guides students to learn about the applicability of peroxidase enzymes to degrade organic dyes (as model pollutants) in simulated waste water. In addition to showing how enzymes can potentially be used for waste water remediation, various factors than can affect enzyme-based reactions such as pH, temperature, concentration of substrates/enzymes, and denaturants can also be tested. This "applied biotechnology" experiment was successfully implemented in an undergraduate biochemistry laboratory course to enhance students' learning of environmental issues as well important biochemistry concepts. Student survey confirmed that this laboratory experiment was successful in achieving the objectives of raising environmental awareness in students and illustrating the usefulness of chemistry in solving real-life problems. This experiment can be easily adopted in an introductory biochemistry laboratory course and taught as an inquiry-guided exercise.
机译：我们的环境不断受到压力，并受到各种污染源的威胁。理科学生，尤其是化学专业的学生，​​不仅必须意识到这些问题，而且还应被告知化学（和科学）可以为此类现实问题提供解决方案。为此，本文介绍了一项新开发的生物化学实验室实验，该实验可指导学生学习过氧化物酶对降解模拟废水中有机染料（作为模型污染物）的适用性。除了显示如何将酶潜在地用于废水修复之外，还可以测试各种可能影响基于酶的反应的因素，例如pH，温度，底物/酶的浓度和变性剂。此“应用生物技术”实验已成功在本科生化学实验室课程中实施，以增强学生对环境问题以及重要生物化学概念的学习。学生调查证实，该实验室实验成功实现了提高学生的环境意识以及说明化学方法在解决现实生活中的问题的目标。该实验可以很容易地在生物化学入门课程中采用，并作为探究指导性练习进行讲授。

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