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首页> 外文期刊>Biochemical Genetics >Over-seasonal and sex-independent expression of kappa casein gene (CSN3) in mammalian blood lymphocytes.
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Over-seasonal and sex-independent expression of kappa casein gene (CSN3) in mammalian blood lymphocytes.

机译：酪蛋白酪蛋白基因（CSN3）在哺乳动物血液淋巴细胞中的季节性和性别独立表达。

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摘要

After observing the nonspecific expression of the alpha S1 casein gene in peripheral blood mononuclear cells of goats, we continued to trace illegitimate gene-expression patterns in mammals. Six mammalian species (Bos taurus, Capra hircus, Ovis aries, Equus caballus, Sus scrofa, and Bison bonasus) were analyzed for the presence of kappa casein in peripheral blood mononuclear cells and Ficoll-isolated lymphocytes. To detect potential relationships between the expression pattern and reproductive status of the individual, the animals within species represented different sex, age, and physiological status (immature and reproducing males; immature, pregnant, lactating, and sterile females). Species-specific primers were designed for the two-step post reverse transcriptional-polymerase chain reaction. The products were sequenced and a Blast search performed. Transcripts of kappa casein were successfully detected in the peripheral blood mononuclear cells and lymphocytes of three of the species (goat, cow, and European bison). The presence of the transcripts was unrelated to sex, physiological status, or age of the animals.
机译：观察山羊外周血单个核细胞中αS1酪蛋白基因的非特异性表达后，我们继续追踪哺乳动物中非法的基因表达模式。分析了六个哺乳动物物种（金牛座，Capra hircus，Ovis aries，马属马，Sus scrofa和野牛鼻）在外周血单核细胞和Ficoll分离的淋巴细胞中是否存在酪蛋白酪蛋白。为了检测个体的表达模式与生殖状态之间的潜在关系，物种内的动物代表不同的性别，年龄和生理状态（未成熟和正在繁殖的雄性;未成熟，怀孕，哺乳和不育的雌性）。针对两步逆转录聚合酶链反应设计了物种特异性引物。对产物进行测序并进行Blast搜索。在三种动物（山羊，牛和欧洲野牛）的外周血单核细胞和淋巴细胞中成功检测到了酪蛋白的转录本。转录本的存在与动物的性别，生理状态或年龄无关。

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