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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >TWO-DIMENSIONAL GEL ELECTROPHORESIS OF NASAL AND BRONCHOALVEOLAR LAVAGE FLUIDS AFTER OCCUPATIONAL EXPOSURE
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TWO-DIMENSIONAL GEL ELECTROPHORESIS OF NASAL AND BRONCHOALVEOLAR LAVAGE FLUIDS AFTER OCCUPATIONAL EXPOSURE

机译：职业性接触后鼻和支气管肺泡灌洗液的二维凝胶电泳

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摘要

Human nasal lavage fluids (NLFs), and bronchoalveolar lavage fluids (BALFs) were analyzed with two-dimensional gel electrophoresis (2-DE) and the protein patterns were evaluated with a computerized imaging 2-DE system. With silver staining about 1000 spots were detected in 10 mu g samples of NLF or BALF. Both BALF and NLF 2-DE patterns showed similarities to a reference plasma pattern and about 25 plasma proteins were identified in NLF as well as in BALF. Comparison showed that the levels of albumin and transferrin appeared to be slightly higher in BALF than NLF, while the levels of IgA, IgG and haptoglobin beta were higher in NLF than in BALF. In contrast to BALF and blood plasma, NLF contained large amounts of a cluster of acidic proteins (pI 4.5-5.5) with molecular masses of 15-30 kDa. Distinct alterations in the NLF 2-DE patterns were found in a worker who developed an asthmatic condition with bronchial hyperreactivity after exposure to organic acid anhydrides. After exposure, 14 protein spots were increased and one decreased by a factor of > 3 as compared to the levels before exposure and compared to healthy individuals. This is the first study indicating that 2-DE of NLF may be used to investigate early changes in airway protein patterns induced by occupational exposure to irritating chemicals. [References: 20]
机译：使用二维凝胶电泳（2-DE）分析人的鼻灌洗液（NLF）和支气管肺泡灌洗液（BALF），并使用计算机成像2-DE系统评估蛋白质模式。用银染色，在10μgNLF或BALF样品中检测到约1000个斑点。 BALF和NLF 2-DE模式均显示与参考血浆模式相似，并且在NLF和BALF中鉴定出约25种血浆蛋白。比较表明，BALF中的白蛋白和转铁蛋白水平似乎略高于NLF，而NLF中的IgA，IgG和触珠蛋白β的水平高于BALF。与BALF和血浆相反，NLF含有大量的酸性蛋白质簇（pI 4.5-5.5），分子量为15-30 kDa。一名工人在暴露于有机酸酐后出现哮喘，支气管反应过度，发现其NLF 2-DE模式有明显变化。暴露后，与健康个体相比，增加了14个蛋白质斑点，减少了一个蛋白质斑点，其比例> 3。这是第一项表明NLF的2-DE可用于研究职业性接触刺激性化学物质引起的气道蛋白质模式早期变化的研究。 [参考：20]

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