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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >THE ASSAY DEVELOPMENT OF A MOLECULAR MARKER FOR TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES
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THE ASSAY DEVELOPMENT OF A MOLECULAR MARKER FOR TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES

机译：传染性海绵状脑病的分子标记物的研究进展

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摘要

The transmissible spongiform encephalopathies are a group of neurodegenerative diseases which include Creutzfeldt-Jakob disease (CJD) in humans, scrapie in sheep, and bovine spongiform encephalopathy (BSE) in cattle. Two-dimensional electrophoresis of proteins was previously used to identify two marker proteins, 130/131, which ate selectively present in the cerebrospinal fluid (CSF) of patients with CJD and not in patients with other dementias. The recent characterization of these proteins by amir-io acid sequencing has identified them as members of the 14-3-3 family of proteins. Polyclonal antibodies against 14-3-3 (all isoforms), 14-3-3 gamma, 14-3-3 beta, and 14-3-3 theta are immunoreactive with a 30 kDa marker band from CJD CSF. Silver Staining of two-dimensional electrophoresis separated BSE CSF proteins does not identify a similar marker, However, 14-3-3 immunoreactivity is found in cattle CSF when these proteins are blotted to polyvinylidene difluoride but not when blotted to nitrocellulose. [References: 11]
机译：可传播的海绵状脑病是一组神经退行性疾病，包括人类的克雅氏病（CJD），绵羊的瘙痒病和牛的牛海绵状脑病（BSE）。蛋白质的二维电泳以前用于鉴定两种标记蛋白130/131，它们选择性地存在于CJD患者的脑脊髓液（CSF）中，而不存在于其他痴呆患者中。这些蛋白质最近通过阿米-碘酸测序的表征已将它们鉴定为14-3-3蛋白质家族的成员。针对14-3-3（所有同种型），14-3-3γ，14-3-3β和14-3-3 theta的多克隆抗体与来自CJD CSF的30 kDa标记带发生免疫反应。二维电泳分离的BSE CSF蛋白的银染未鉴定出类似的标记物，但是，当牛CSF中的蛋白被二聚偏二氟乙烯印迹而被硝化纤维素印迹时，则发现14-3-3免疫反应性。 [参考：11]

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