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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Comparative study of floating and dynamic injection modes in electrokinetic separative microsystems
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Comparative study of floating and dynamic injection modes in electrokinetic separative microsystems

机译：电动分离微系统中浮动和动态注入模式的比较研究

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摘要

Miniaturization of analytical instruments has attracted a wide interest in analytical chemistry over the past decade because of the advantages of reduced reagent consumption, better analytical performance, and shorter analysis time. The widespread interest in this field has resulted in efforts to develop chips. For chips involving separation, injection is a key step to achieve efficient and sensitive analysis. This work presents a comparative study of two electrokinetic injection modes in chips: the floating, which has been mainly used up to now, and the dynamic. This study was done with a crossjunction, either with numerical simulations or with experiments. Experiments were carried out with homemade PDMS-glass microsystems involving zonal electrophoresis analysis of five derivatized amino acids. Injected amount, reproducibility, separation efficiency, and analyte discrimination were evaluated and discussed. The experimental results were successfully correlated with numerical simulations. It appeared that the dynamic injection mode is much more appropriate than the floating mode as it is faster (reduction by a factor 2 of the total analysis time here), more reproducible (RSD of peak areas equal to 1.3% (n = 4) instead of 10% (n = 4)), and leads to more efficient separation (about 20% with 3 cm separation channel length) for the same injected amount, whatever the amount, because the sample plug is less dispersed.
机译：在过去的十年中，由于减少了试剂消耗，提高了分析性能并缩短了分析时间，分析仪器的小型化引起了分析化学的广泛兴趣。在该领域的广泛兴趣导致了开发芯片的努力。对于涉及分离的碎片，进样是实现高效和灵敏分析的关键步骤。这项工作对芯片中的两种电动注入模式进行了比较研究：浮子（迄今为止主要使用）和动态。这项研究是通过交叉连接进行的，无论是数值模拟还是实验。实验是使用自制的PDMS玻璃微系统进行的，涉及五个衍生氨基酸的区域电泳分析。评估并讨论了进样量，重现性，分离效率和分析物区分度。实验结果已成功地与数值模拟相关联。似乎动态注入模式比浮动模式更合适，因为它更快（此处减少了总分析时间的2倍），可重现性更高（峰面积的RSSD等于1.3％（n = 4））。 10％（n = 4）），并且无论进样量如何，对于相同的进样量，分离效率都更高（对于3 cm的分离通道长度，分离效率约为20％），因为样品塞的分散程度较小。

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