PREFRACTIONATION OF PROTEIN SAMPLES PRIOR TO TWO-DIMENSIONAL ELECTROPHORESIS

Corthals GL.; Herbert BR.; Williams KL.; Gooley AA.; Molloy MP.

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PREFRACTIONATION OF PROTEIN SAMPLES PRIOR TO TWO-DIMENSIONAL ELECTROPHORESIS

机译：二维电泳前蛋白质样品的预提取

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Thousands of proteins may be visualised on a two-dimensional (2-D) gel, but only hundreds are present at levels sufficient for chemical analysis. Therefore, prefractionation of protein samples prior to 2-D polyacrylamide gel electrophoresis (PAGE) will be important for the investigation of proteins that are present at sub-picogram levels in physiological samples. We describe an approach to prefractionate protein samples prior to 2-D PAGE using the Gradiflow, which is a new (preparative) electrokinetic membrane apparatus designed to fractionate proteins in a number of different ways. We have fractionated human serum under nonreducing conditions using the 'reflux' mode, in which proteins are fractionated according to their relative mobility under controlled electrophoretic conditions, where the current is periodically reversed. We describe how fractionation occurs and present examples of enrichment of specific proteins. [References: 31]

机译：可以在二维（2-D）凝胶上可视化成千上万种蛋白质，但仅存在数百种，足以进行化学分析。因此，在2-D聚丙烯酰胺凝胶电泳（PAGE）之前对蛋白质样品进行预分级分离对于研究生理样品中亚皮克水平上存在的蛋白质至关重要。我们描述了一种使用Gradiflow在2-D PAGE之前对蛋白质样品进行预分离的方法，该方法是一种旨在以多种不同方式分离蛋白质的新型（制备）电动膜设备。我们已使用“回流”模式在非还原条件下分离了人类血清，其中蛋白质在受控的电泳条件下根据其相对迁移率进行分离，其中电流会定期反向。我们描述了分级分离的发生方式，并介绍了富集特定蛋白质的实例。 [参考：31]

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《Electrophoresis: The Official Journal of the International Electrophoresis Society》 |1997年第4期|共7页
作者
Corthals GL.; Herbert BR.; Williams KL.; Gooley AA.; Molloy MP.;
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正文语种 eng
中图分类化学;
关键词
Prefractionation; Preparative electrophoresis; Native electrophoresis; Two-dimensional polyacrylamide gel electrophoresis; Proteome; Immobilized ph gradients; 2-dimensional electrophoresis; Database; Sequence;

机译：预分离;制备电泳;本征电泳;二维聚丙烯酰胺凝胶电泳;蛋白质组学;固定化的pH梯度;二维电泳;数据库;序列;

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机译：二维凝胶电泳之前，基于等电点的粗制生物样品中蛋白质的蛋白质初步分离。
2. Reversed-phase high-performance liquid chromatography prefractionation prior to two-dimensional difference gel electrophoresis and mass spectrometry identifies new differentially expressed proteins between striate cortex of kitten and adult cat [J] . Van den Bergh G., Clerens S., Vandesande F., Electrophoresis: The Official Journal of the International Electrophoresis Society . 2003,第9期

机译：二维差异凝胶电泳和质谱分析前的反相高效液相色谱法预分离可鉴定小猫和成年猫的纹状皮层之间新的差异表达蛋白
3. Towards global analysis of mammalian proteomes using sample prefractionation prior to narrow pH range two-dimensional gels and using one-dimensional gels for insoluble and large proteins [J] . Zuo X., Hembach P., Tang HY., Electrophoresis: The Official Journal of the International Electrophoresis Society . 2001,第9期

机译：要在狭窄的pH范围内使用样品预分馏二维凝胶，对不溶和大蛋白使用一维凝胶，对哺乳动物蛋白质组进行全局分析
4. An effective skeletal muscle prefractionation method to remove abundant structural proteins for optimized two-dimensional gel electrophoresis [C] . Bradley Jarrold, Jeffrey DeMuth, Kenneth Greis International Symposium on Runaway Reactions, Pressure Relief Design and Effluent Handling American Institute of Chemical Engineers/American Chemical Society Management Conference . 2005

机译：一种有效的骨骼肌预选方法，去除优化的二维凝胶电泳优化的结构蛋白
5. Systematic studies of horseshoe crabs, land snails, and freshwater fish by two-dimensional electrophoresis of proteins [D] . Miyazaki Junichi, Miyazaki Jun-ichi 1990

机译：通过蛋白质的二维电泳系统研究studies，田螺和淡水鱼
6. Sample complexity reduction for two-dimensional electrophoresis using solution isoelectric focusing prefractionation [O] . Matthew R. Richardson, Sean Liu, Heather N. Ringham, -1

机译：使用溶液等电聚焦预分离技术降低二维电泳的样品复杂度
7. Validation of a prefractionation method followed by two-dimensional electrophoresis – Applied to cerebrospinal fluid proteins from frontotemporal dementia patients [O] . Hansson Sara Folkesson, Puchades Maja, Blennow Kaj, 2004

机译：预分离方法的验证，然后进行二维电泳–用于额颞叶痴呆患者的脑脊液蛋白

PREFRACTIONATION OF PROTEIN SAMPLES PRIOR TO TWO-DIMENSIONAL ELECTROPHORESIS

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