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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >HIGH SENSITIVITY IDENTIFICATION OF PROTEINS BY ELECTROSPRAY IONIZATION TANDEM MASS SPECTROMETRY - INITIAL COMPARISON BETWEEN AN ION TRAP MASS SPECTROMETER AND A TRIPLE QUADRUPOLE MASS SPECTROMETER
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HIGH SENSITIVITY IDENTIFICATION OF PROTEINS BY ELECTROSPRAY IONIZATION TANDEM MASS SPECTROMETRY - INITIAL COMPARISON BETWEEN AN ION TRAP MASS SPECTROMETER AND A TRIPLE QUADRUPOLE MASS SPECTROMETER

机译：电喷雾电离串联质谱法高灵敏度鉴定蛋白质-离子阱质谱与三重四极质谱的初步比较。

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摘要

Recently, we have shown that a solid-phase-microextraction/capillary electrophoresis device coupled to an electrospray ionization triple quadrupole mass spectrometer through a microelectrospray interface represents a powerful analytical system for the rapid, conclusive and sensitive identification of proteins separated by gel electrophoresis. Here we report on the successful coupling of the same device to an electrospray ionization ion trap mass spectrometer and on the comparative evaluation of the performance of the triple quadrupole and ion-trap-based systems, In the ion trap mass spectrometer-based system, using a tryptic digest of a calibrated bovine serum albumin sample, we achieved limits of detection in the single mass spectrometry (MS) and tandem MS mode, respectively, of 400 amol (if 20 mu L of solution at a concentration of 20 amol/mu L was applied). The system mras also successfully used to identify six yeast proteins isolated from a single analytical two-dimensional polyacrylamide gel. For the detection of unfragmented peptide ions both systems showed comparable sensitivity, whereas the ion-trap-based system showed superior performance with fragment ion spectra. [References: 31]
机译：最近，我们已经表明，通过微电喷雾接口与电喷雾电离三重四极杆质谱仪耦合的固相微萃取/毛细管电泳装置代表了一种功能强大的分析系统，可快速，结论性和灵敏地鉴定通过凝胶电泳分离的蛋白质。在这里，我们报告了将同一设备成功耦合到电喷雾电离离子阱质谱仪上的情况，并报告了基于离子阱质谱仪的三重四极杆和基于离子阱的系统的性能的比较评估。校准的牛血清白蛋白样品的胰蛋白酶消化产物，我们在单质谱（MS）和串联MS模式下分别达到400 amol的检测限（如果20μL的溶液浓度为20 amol /μL已应用）。该系统还成功地用于鉴定从单一分析型二维聚丙烯酰胺凝胶分离出的六种酵母蛋白。对于检测未片段化的肽离子，两个系统均显示出可比的灵敏度，而基于离子阱的系统在碎片离子谱图中显示出优异的性能。 [参考：31]

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