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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >The effects of nanoparticles uptaken by cells on electrorotation.
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The effects of nanoparticles uptaken by cells on electrorotation.

机译：细胞摄取的纳米颗粒对电旋转的影响。

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Electrorotation (ER) has become a very powerful diagnostic technique for the measurement of dielectric properties of cells. However, only a few papers have investigated the electric-induced rotation of particles in a stationary alternating (AC) electric field instead of a rotating electric field. In this study, a microchip composed of a top-grounded electrode, flow chamber and bottom chess-type electrode arrays was used to construct a stationary non-uniform AC electric field for the manipulation of cells by dielectrophoretic force. We focused on the effects of metal and dielectric nanoparticles uptaken by cells under ER, by using human promyelocytic leukemia cells (HL-60), 13 nm Au and 19 nm SiO(2) nanoparticles. As revealed by the experimental results, both the percentage of cells in rotation and the range of rotational (ROT) frequency for the uptake of Au nanoparticle cells were higher and wider than in the case of SiO(2) nanoparticles. In addition, the rotation of lone cells and pearl-chain cells under non-uniform and uniform electric field were quantitatively investigated, respectively. The membrane capacitance and membrane conductance of HL-60 cells can be extracted from the ROT spectra as 10.18+/-1.92 mF/m(2) and 1500+/-321 S/m(2), respectively. In general, the ER of cells in a stationary AC electric field can be attributed to the highly non-uniform electric field and non-uniform dispersion of nanoparticles within cells; therefore, the electrical properties of uptaken nanoparticles and the aggregation phenomenon have significant influences on the resulting electrical torque.
机译：电旋转（ER）已成为用于测量细胞介电特性的非常强大的诊断技术。但是，只有少数几篇论文研究了在固定交流（AC）电场而不是旋转电场中粒子的电感应旋转。在这项研究中，由顶部接地的电极，流动室和底部象棋型电极阵列组成的微芯片用于构建固定的非均匀交流电场，以通过介电泳力操纵细胞。通过使用人早幼粒细胞白血病细胞（HL-60），13 nm Au和19 nm SiO（2）纳米颗粒，我们集中研究了在ER下细胞摄取的金属和介电纳米颗粒的影响。从实验结果可以看出，吸收Au纳米颗粒细胞的旋转细胞百分比和旋转（ROT）频率范围均比SiO（2）纳米颗粒更高和更宽。另外，分别定量研究了非均匀电场和均匀电场作用下孤细胞和珍珠链细胞的旋转。 HL-60细胞的膜电容和膜电导可以从ROT光谱中分别提取为10.18 +/- 1.92 mF / m（2）和1500 +/- 321 S / m（2）。通常，在固定交流电场下细胞的ER可以归因于纳米粒子在细胞内的高度不均匀电场和不均匀分散。因此，摄取的纳米粒子的电特性和聚集现象对所得的电转矩具有重大影响。

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