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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Selective concentration of human cancer cells using contactless dielectrophoresis.
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Selective concentration of human cancer cells using contactless dielectrophoresis.

机译：使用非接触介电电泳选择性浓缩人类癌细胞。

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摘要

This work is the first to demonstrate the ability of contactless dielectrophoresis (cDEP) to isolate target cell species from a heterogeneous sample of live cells. Since all cell types have a unique molecular composition, it is expected that their dielectrophoretic (DEP) properties are also unique. cDEP is a technique developed to improve upon traditional and insulator-based DEP devices by replacing embedded metal electrodes with fluid electrode channels positioned alongside desired trapping locations. Through the placement of the fluid electrode channels and the removal of contact between the electrodes and the sample fluid, cDEP mitigates issues associated with sample/electrode contact. MCF10A, MCF7, and MDA-MB-231 human breast cells were used to represent early, intermediate, and late-staged breast cancer, respectively. Trapping frequency responses of each cell type were distinct, with the largest difference between the cells found at 20 and 30 V. MDA-MB-231 cells were successfully isolated from a population containing MCF10A and MCF7 cells at 30 V and 164 kHz. The ability to selectively concentrate cells is the key to development of biological applications using DEP. The isolation of these cells could provide a workbench for clinicians to detect transformed cells at their earliest stage, screen drug therapies prior to patient treatment, increasing the probability of success, and eliminate unsuccessful treatment options.
机译：这项工作是首次证明非接触介电电泳（cDEP）从活细胞异质样品中分离靶细胞物种的能力。由于所有细胞类型均具有独特的分子组成，因此预期它们的介电电泳（DEP）特性也是独特的。 cDEP是一种通过用位于所需捕集位置旁边的流体电极通道代替嵌入式金属电极来改进传统和基于绝缘体的DEP设备的技术。通过放置流体电极通道以及去除电极与样品流体之间的接触，cDEP减轻了与样品/电极接触相关的问题。 MCF10A，MCF7和MDA-MB-231人乳腺细胞分别代表早期，中期和晚期乳腺癌。每种细胞类型的诱捕频率响应是截然不同的，在20和30 V时发现的细胞之间的差异最大。从30 V和164 kHz的包含MCF10A和MCF7细胞的种群中成功分离出MDA-MB-231细胞。选择性浓缩细胞的能力是使用DEP开发生物应用的关键。这些细胞的分离可以为临床医生提供一个工作台，以在其最早阶段检测转化的细胞，在患者治疗之前筛选药物疗法，增加成功的可能性，并消除不成功的治疗选择。

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