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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Elucidating the identity of Anisakis larvae from a broad range of marine fishes from the Yellow Sea, China, using a combined electrophoretic-sequencing approach.
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Elucidating the identity of Anisakis larvae from a broad range of marine fishes from the Yellow Sea, China, using a combined electrophoretic-sequencing approach.

机译：使用组合的电泳测序方法，阐明了来自中国黄海的多种海洋鱼类中的Anisakis幼虫的身份。

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摘要

Larval nematodes were collected from marine fishes from the Yellow Sea, China. Specimens (n=1731) of Anisakis type I from 311 fishes (representing 40 species) were each identified based on morphological characters. From the genomic DNA from individual specimens, a region of nuclear ribosomal DNA was amplified by PCR, followed by digestion with restriction endonuclease HinfI, TaqI or HhaI. Subsequently, the ITS-1 and ITS-2 regions of selected samples were sequenced. The results revealed three species of Anisakis, namely Anisakis pegreffii (n=1709), A. typica (n=3) and a genotype (n=19) proposed, also based on comparison with previous studies, to be a "hybrid" between A. pegreffii and A. simplex sensu stricto. Thus, A. pegreffii was the dominant species, accounting for 98.7% of the total number of specimens examined herein. This is the first report of A. typica and the "hybrid" genotype from fishes from the Yellow Sea. This study provides important basic information on Anisakis in this region and suggests that the genus Anisakis has substantial host and geographical distributions.
机译：幼虫线虫是从中国黄海的海水鱼类中采集的。根据形态学特征，从311种鱼类（代表40种）中鉴定出I型Anisakis I型标本（n = 1731）。从单个标本的基因组DNA中，通过PCR扩增核糖体DNA区域，然后用限制性核酸内切酶HinfI，TaqI或HhaI消化。随后，对所选样品的ITS-1和ITS-2区域进行测序。结果显示，与以前的研究相比较，也基于与先前研究的比较，提出了三种Anisakis物种，即Anisakis pegreffii（n = 1709），A。typica（n = 3）和提出的基因型（n = 19）。 A. pegreffii和A. simplex sensu stricto。因此，A。pegreffii是优势种，占本文检查的标本总数的98.7％。这是A. typica和黄海鱼类“杂种”基因型的首次报道。这项研究提供了该地区Anisakis的重要基础信息，并表明Anisakis属具有大量寄主和地理分布。

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