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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Identification of methylated regions with peak search based on Poisson model from massively parallel methylated DNA immunoprecipitation-sequencing data.
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Identification of methylated regions with peak search based on Poisson model from massively parallel methylated DNA immunoprecipitation-sequencing data.

机译：基于大规模平行甲基化DNA免疫沉淀测序数据基于Poisson模型的峰搜索来鉴定甲基化区域。

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摘要

DNA methylation is one of the most important epigenetic modification types, which plays a critical role in gene expression. High efficient surveying of whole genome DNA methylation has been aims of many researchers for long. Recently, the rapidly developed massively parallel DNA-sequencing technologies open the floodgates to vast volumes of sequence data, enabling a paradigm shift in profiling the whole genome methylation. Here, we describe a strategy, combining methylated DNA immunoprecipitation sequencing with peak search to identify methylated regions on a whole-genome scale. Massively parallel methylated DNA immunoprecipitation sequencing combined with methylation DNA immunoprecipitation was adopted to obtain methylated DNA sequence data from human leukemia cell line K562, and the methylated regions were identified by peak search based on Poison model. From our result, 140 958 non-overlapping methylated regions have been identified in the whole genome. Also, the credibility of result has been proved by its strong correlation with bisulfite-sequencing data (Pearson R(2)=0.92). It suggests that this method provides a reliable and high-throughput strategy for whole genome methylation identification.
机译：DNA甲基化是最重要的表观遗传修饰类型之一，其在基因表达中起关键作用。长期以来，高效研究全基因组DNA甲基化一直是许多研究人员的目标。最近，快速发展的大规模并行DNA测序技术打开了通往大量序列数据的闸门，从而在对整个基因组甲基化进行分析时实现了范式转变。在这里，我们描述了一种策略，将甲基化的DNA免疫沉淀测序与峰搜索相结合，以识别全基因组规模上的甲基化区域。采用大规模平行甲基化DNA免疫沉淀测序结合甲基化DNA免疫沉淀的方法从人白血病细胞株K562中获得甲基化DNA序列数据，并基于Poison模型通过峰搜索确定甲基化区域。根据我们的结果，已在整个基因组中鉴定出140958个不重叠的甲基化区域。此外，结果的可信度已通过其与亚硫酸氢盐测序数据的强相关性得到证明（Pearson R（2）= 0.92）。这表明该方法为全基因组甲基化鉴定提供了可靠且高通量的策略。

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