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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >CONSTRUCTION OF HSC-2DPAGE - A TWO-DIMENSIONAL GEL ELECTROPHORESIS DATABASE OF HEART PROTEINS
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CONSTRUCTION OF HSC-2DPAGE - A TWO-DIMENSIONAL GEL ELECTROPHORESIS DATABASE OF HEART PROTEINS

机译：HSC-2DPAGE的构建-二维凝胶蛋白电泳蛋白质数据库

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The dissemination of information relating to the characterisation of proteins from two-dimensional electrophoresis (2-DE) gel databases is essential for their effective utilisation in the study of protein expression in cell biology. Since the inception of the World Wide Web and the pioneering development of SWISS-2DPAGE as a tool for retrieving information on proteins separated by 2-DE, the Internet has become the method of choice for disseminating and accessing information on 2-DE protein databases. At Harefield we have established HSC-2DPAGE which is an advanced interface for accessing protein databases relating to heart disease. The Web site currently includes databases of proteins from human, dog and rat ventricular tissue and a human endothelial cell line. The databases are searchable individually or as a whole by remote keyword searches. Each database is represented by both synthetic (computer generated) and real (scanned gel) clickable images upon which characterised protein spots are highlighted by hyperlinked symbols. The database conforms to all the rules proposed for federated 2-DE protein databases and individual protein entries are linked to other protein databases such as SWISS-PROT by active cross-references. This paper describes the construction of HSC-2DPAGE, its maintenance, and access via the Internet. [References: 26]
机译：二维电泳（2-DE）凝胶数据库中与蛋白质表征有关的信息传播对于有效利用它们在细胞生物学中研究蛋白质表达至关重要。自从互联网问世以来，SWISS-2DPAGE作为检索由2-DE分离的蛋白质的信息的工具的开创性发展，互联网已经成为在2-DE蛋白质数据库上传播和访问信息的首选方法。在Harefield，我们建立了HSC-2DPAGE，它是访问与心脏病有关的蛋白质数据库的高级界面。该网站目前包括来自人，狗和大鼠心室组织以及人内皮细胞系的蛋白质数据库。可以通过远程关键字搜索来单独或整体搜索数据库。每个数据库均由可合成的（计算机生成的）和真实的（扫描的凝胶）可点击图像表示，在图像上通过超链接符号突出显示了特征蛋白点。该数据库符合针对联合2-DE蛋白质数据库提出的所有规则，并且各个蛋白质条目通过主动交叉引用链接到其他蛋白质数据库，例如SWISS-PROT。本文介绍了HSC-2DPAGE的结构，其维护以及通过Internet的访问。 [参考：26]

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