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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Explorations of ABO-Rh antigen expressions on erythrocyte dielectrophoresis: changes in cross-over frequency.
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Explorations of ABO-Rh antigen expressions on erythrocyte dielectrophoresis: changes in cross-over frequency.

机译：红细胞介电泳上ABO-Rh抗原表达的探索：交叉频率的变化。

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摘要

A quadrupole dielectrophoretic microdevice was utilized to examine the ABO-Rh dependencies on erythrocyte polarizations. This important step toward medical microdevice technology would transform key clinical blood tests from the laboratory into the field. Previous work in dielectrophoretic microdevices demonstrated that the large number of ABO antigens on erythrocyte membranes impacts their dielectrophoretic signature at 1 MHz. This work explores the dielectrophoretic behavior of native human erythrocytes categorized by their ABO-Rh blood types and directly compares these responses to the same erythrocyte sample modified to remove the A and B antigens. A beta(1-3)-galactosidase enzyme was utilized to cleave the ABO polysaccharide backbone at the galactosidase bonds. The enzymatic reaction was optimized by comparing agglutination of the native and modified blood cells in addition to UV-Vis and HPLC analysis of the reaction effluent for saccharide residues. Next, the dielectrophoretic behaviors of the native and modified erythrocytes were visually verified in a quadrupole electrode microdevice over a frequency range from 100 kHz to 80 MHz. The lower cross-over frequency (COF), which transitions from negative to positive dielectrophoresis, for ABO blood types tested (A+, A-, B+, B-, AB+, O+ and O-) differed over the range from 17 to 47 MHz. The COFs of the corresponding enzyme-modified erythrocytes were also determined and the range narrowed to 29-41 MHz. A second COF in the 70-80 MHz range was observed and was reduced in the presence of the transmembrane Rhesus factor. These results suggest that antigen expression on erythrocyte membrane surfaces influence cell polarizations in nonuniform AC fields.
机译：利用四极介电泳微器件检查ABO-Rh对红细胞极化的依赖性。迈向医疗微设备技术的这一重要步骤将把关键的临床血液测试从实验室转变为现场。介电电泳微器件的先前工作表明，红细胞膜上的大量ABO抗原会在1 MHz时影响其介电电泳特征。这项工作探索了按其ABO-Rh血型分类的天然人类红细胞的介电泳行为，并将这些反应与经过修饰以去除A和B抗原的相同红细胞样品进行直接比较。使用β（1-3）-半乳糖苷酶在半乳糖苷酶键处裂解ABO多糖主链。通过比较天然和修饰的血细胞的凝集以及UV-Vis和HPLC分析反应流出物中糖类残留物的方法，优化了酶促反应。接下来，在四极电极微器件中从100 kHz到80 MHz的频率范围内目视验证了天然和修饰的红细胞的介电泳行为。对于所测试的ABO血型（A +，A-，B +，B-，AB +，O +和O-），较低的交叉频率（COF）从负介电电泳转变为正介电电泳。还确定了相应酶修饰的红细胞的COF，范围缩小到29-41 MHz。观察到第二个COF在70-80 MHz范围内，并在存在跨膜恒河猴因子的情况下降低。这些结果表明，红细胞膜表面上的抗原表达会影响非均匀交流电场中的细胞极化。

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