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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Polydopamine as an adhesive coating for open tubular capillary electrochromatography.
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Polydopamine as an adhesive coating for open tubular capillary electrochromatography.

机译：聚多巴胺作为用于开放式管状毛细管电色谱的粘合剂涂层。

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摘要

Polydopamine (PolyD) coating was used as an adhesive layer in the preparation of biological stationary phases for open tubular capillary electrochromatography (OT-CEC). The influence of coating solution freshness, coating time, temperature and dopamine hydrochloride concentration on the PolyD layer formation was studied. The performance of the polyD coating was monitored by measuring the electro-osmotic flow in coated capillaries. Following polyD coating of the capillary, secondary layer material (e.g. cell membrane solutions, phospholipid mixtures or mitochondria) was inserted into the capillary for at least 1 h. The performance of these double-coated capillaries (a polyD layer+a biological material layer) was compared with capillaries containing the respective biological material directly attached to the capillary wall. The study reveals that the presence of polyD layer in fused silica capillaries improves the performance of lipid and membrane fragment coatings in capillaries. At the same time, the thickness of the polyD layer does not have marked impact on the secondary coatings. Analysis with test analytes demonstrated that double-coated capillaries can be applied to study membrane-drug interactions.
机译：聚多巴胺（PolyD）涂层在制备用于开放式管状毛细管电色谱（OT-CEC）的生物固定相中用作粘合层。研究了包衣溶液的新鲜度，包衣时间，温度和盐酸多巴胺浓度对PolyD层形成的影响。通过测量涂层毛细管中的电渗流来监测polyD涂层的性能。在对毛细管进行PolyD涂层后，将第二层材料（例如细胞膜溶液，磷脂混合物或线粒体）插入毛细管至少1小时。将这些双涂层毛细管（polyD层+生物材料层）的性能与包含直接附着在毛细管壁上的相应生物材料的毛细管进行了比较。研究表明，熔融石英毛细管中存在polyD层可改善毛细管中脂质和膜碎片涂层的性能。同时，polyD层的厚度对辅助涂层没有明显的影响。用测试分析物进行的分析表明，双层毛细管可用于研究膜与药物的相互作用。

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