...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Electrokinetic injection of samples into a short electrophoretic capillary controlled by piezoelectric micropumps
【24h】

Electrokinetic injection of samples into a short electrophoretic capillary controlled by piezoelectric micropumps

机译：将样品电动注入到由压电微型泵控制的短电泳毛细管中

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Electrokinetic sample injection using two piezoelectric micropumps has been proposed for electrophoresis in short capillaries. The sample is brought to the injection end of the capillary using one of them. Then, the high-voltage source is turned on and the sample is injected electrokinetically for a defined time. The injection is terminated by removal of the sample zone by the flowing separation electrolyte pumped by the second piezoelectric micropump. The RSD value, expressing the repeatability of the injection, does not exceed 4%. The injection apparatus does not contain any mobile mechanical components, there is no movement of the capillary and both its ends remain constantly in the solution during both the sample injection and separation. Thus, the micropumps replace the six-way injection valve and linear pump in similar types of injection apparatuses. The injection was tested in the separation and determination of ammonium and potassium ions in two samples of mineral fertilizers. The separation was performed in background electrolyte containing 500 mM of acetic acid +20mM Tris + 2mM 18-crown-6 ( pH 3.3) in a capillary with id 50 mu m and total length/length to the contactless conductivity detector of 10.5/8 cm. The injection and separation took place at a voltage of 5 kV and the separation time equaled 20 s. The measured values of the analyte contents corresponded to the value declared by the manufacturer within the reliability interval, where RSD equaled between 3.5 and 4.7%.
机译：已经提出了使用两个压电微型泵进行电动样品注射以用于短毛细管电泳。使用其中之一将样品带入毛细管的注射端。然后，打开高压电源，并在规定的时间内电动注入样品。通过由第二压电微型泵泵送的流动的分离电解质去除样品区域，终止注入。表示进样重复性的RSD值不超过4％。进样设备不包含任何可移动的机械组件，毛细管没有移动，并且其两端在样品进样和分离过程中始终保持在溶液中。因此，在相似类型的注射装置中，微型泵代替了六通注射阀和线性泵。在两种矿物肥料样品中分离和测定铵离子和钾离子时，对注射剂进行了测试。分离是在含有500 mM乙酸+ 20mM Tris + 2mM 18-crown-6（pH 3.3）的毛细管中在50μm的毛细管中进行的，该溶液的总长度/长度为10.5 / 8 cm的非接触电导率检测器。注入和分离在5 kV的电压下进行，分离时间等于20 s。分析物含量的测量值对应于制造商在可靠性区间内声明的值，其中RSD介于3.5％和4.7％之间。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号