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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Evaluation of the free radical scavenging capability of wheat extracts by capillary electrophoresis and multivariate curve resolution.
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Evaluation of the free radical scavenging capability of wheat extracts by capillary electrophoresis and multivariate curve resolution.

机译：通过毛细管电泳和多元曲线分辨率评估小麦提取物的自由基清除能力。

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摘要

In the current work, phenolic acids were analysed and their radical scavenging activity evaluated by CE, including the quantification of overlapping peaks by applying the multivariate curve resolution (MCR) method. To investigate radical scavenging activity, the solution of phenolic acids was treated with a stable free 2,2-diphenyl-1-picrylhydrazyl radical of different concentrations and the radical scavenging activity of the reaction mixture was monitored. The radical scavenging reaction was also applied to wheat bran extracts. The overlapping peaks on the electropherograms of both mixtures of standard compounds and extracts of different wheat bran varieties were resolved by using the MCR method, assuming that the overlapping compounds have different UV spectra. MCR enabled the insufficient resolution between syringic and ferulic acids to be effectively resolved.
机译：在当前的工作中，分析了酚酸，并通过CE评估了其清除自由基的活性，包括通过应用多元曲线分辨率（MCR）方法对重叠峰进行定量。为了研究自由基清除活性，将酚酸溶液用不同浓度的稳定的游离2，2-二苯基-1-吡啶并肼基进行处理，并监测反应混合物的自由基清除活性。自由基清除反应也适用于麦麸提取物。假设重叠的化合物具有不同的UV光谱，则使用MCR方法解析标准化合物混合物和不同麦麸品种的提取物的电泳图上的重叠峰。 MCR使丁香酸和阿魏酸之间的分离度不足得以有效解决。

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