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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Dielectrophoretic concentrator enhancement based on dielectric poles for continuously flowing samples
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Dielectrophoretic concentrator enhancement based on dielectric poles for continuously flowing samples

机译：基于介电极的介电泳浓缩器增强，用于连续流动的样品

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摘要

We describe a novel continuous-flow cell concentrator microdevice based on dielectrophoresis, and its associated custom-made control unit. The performances of a classical interdigitated metal electrode-based dielectrophoresis microfluidic device and this enhanced version, that includes insulator-based pole structures, were compared using the same setup. Escherichia coli samples were concentrated at several continuous flows and the device's trapping efficiencies were evaluated by exhaustive cell counts. Our results show that pole structures enhance the retention up to 12.6%, obtaining significant differences for flow rates up to 20 mu L/min, when compared to an equivalent classical interdigitated electrodes setup. In addition, we performed a subsequent proteomic analysis to evaluate the viability of the biological samples after the long exposure to the actuating electrical field. No Escherichia coli protein alteration in any of the two systems was observed.
机译：我们描述了一种基于介电电泳的新型连续流动细胞浓缩器微装置及其相关的定制控制单元。使用相同的设置比较了基于经典叉指式金属电极的介电泳微流体设备和此增强版本（包括基于绝缘子的电极结构）的性能。大肠埃希氏菌样品以数个连续流进行浓缩，并通过详尽的细胞计数来评估设备的捕集效率。我们的结果表明，与等效的经典叉指式电极设置相比，电极结构可将保留率提高至12.6％，在流速高达20μL / min时获得显着差异。此外，我们进行了后续的蛋白质组学分析，以评估长时间暴露于驱动电场后生物样品的生存能力。在两个系统的任何一个中均未观察到大肠杆菌蛋白质改变。

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