Requirements for the application of protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis and randomly amplified polymorphic DNA analyses to product speciation

Martinez I.; Seppola M.; Friis TJ.

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Requirements for the application of protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis and randomly amplified polymorphic DNA analyses to product speciation

机译：蛋白质十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和随机扩增多态性DNA分析应用于产品形态的要求

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Raw, cooked, fried, smoked and gravad (brine-cured) products were analyzed by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins and by randomly amplified polymorphic DNA (RAPD) in order to identify the species used in their manufacture. The discriminatory power of SDS-PAGE was dependent primarily on the composition and secondarily on the size of the gels: the Laemmli buffer system with 15% acrylamide and 0.087% piperazine diacrylamide separating gels resolved more discriminant protein bands than any of the commercial gels tested. Some of the processing conditions induced alterations in the protein patterns that made identification dubious. Differentiation even between closely related species was easier by RAPD than by SDS-PAGE. Neither the processing conditions nor the tissue from which the DNA was extracted had a significant effect on the RAPD profiles. For identifications based on SDS-PAGE, one should use an optimized gel composition and separate the sample under analysis in the same gel as the references. For RAPD-based identifications, the unknown sample should be amplified together with reference samples and separated in the same gel. [References: 37]

机译：通过蛋白质的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和随机扩增的多态性DNA（RAPD）分析生，熟，炸，熏和格拉达（卤味）产品，以鉴定其所用的物种制造。 SDS-PAGE的辨别能力主要取决于凝胶的组成，其次取决于凝胶的大小：含有15％丙烯酰胺和0.087％哌嗪二丙烯酰胺分离凝胶的Laemmli缓冲系统比任何测试的商业凝胶都能分辨更多的判别蛋白条带。一些加工条件诱导了蛋白质图案的改变，从而使鉴定变得不确定。 RAPD甚至比SDS-PAGE都更容易区分甚至密切相关的物种。加工条件和提取DNA的组织均未对RAPD谱产生显着影响。对于基于SDS-PAGE的鉴定，应使用优化的凝胶组成，并在与参考相同的凝胶中分离待分析样品。对于基于RAPD的鉴定，未知样品应与参考样品一起扩增，并在同一凝胶中分离。 [参考：37]

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《Electrophoresis: The Official Journal of the International Electrophoresis Society》 |2001年第8期|共8页
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Martinez I.; Seppola M.; Friis TJ.;
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1. Requirements for the application of protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis and randomly amplified polymorphic DNA analyses to product speciation [J] . Martinez I., Seppola M., Friis TJ. Electrophoresis: The Official Journal of the International Electrophoresis Society . 2001,第8期

机译：蛋白质十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和随机扩增多态性DNA分析应用于产品形态的要求
2. Speciation and subcellular location of Se-containing proteins in human liver studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and hydride generation-atomic fluorescence spectrometric detection [J] . Chunying Chen, Jiujiang Zhao, Peiqun Zhang, Analytical and bioanalytical chemistry . 2002,第3期

机译：十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和氢化物发生-原子荧光光谱法研究人肝中含硒蛋白的形态和亚细胞定位
3. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and random amplified polymorphic DNA (RAPD) based genetic variation studies in eri silkworm (Samia cynthia ricini Lepidoptera: Saturniidae) [J] . H. R. Singh, B. G. Unni, K. Neog, African Journal of Biotechnology . 2011,第70期

机译：基于十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）和基于随机扩增多态性DNA（RAPD）的蚕的遗传变异研究（Samia cynthia ricini Lepidoptera：Saturniidae）
4. COMPARISON OF AN AUTOMATED RIBOTYPING SYSTEM, PULSED-FIELD GEL ELECTROPHORESIS AND RANDOMLY AMPLIFIED POLYMORPHIC DNA FINGERPRINTING FOR GENOTYPING STREPTOCOCCUS UBERIS [C] . B.E. Gillespie, S.P. Oliver Annual Meeting of the National Mastitis Council . 2003

机译：基因分型链球菌的自动化玻璃型系统，脉冲场凝胶电泳和随机扩增多态性DNA指纹纹理的比较
5. A NOVEL APPROACH TO PROTEIN SEQUENCING BASED ON PARTIAL SPECIFIC CLEAVAGE OF END-LABELED PROTEINS AND ANALYSIS ON SODIUM DODECYL SULFATE POLYACRYLAMIDE GEL ELECTROPHORESIS (BIOCHEMISTRY) [D] . JUE, RODNEY ALAN 1984

机译：基于末端特定蛋白质部分裂解的蛋白质序列分离新方法及十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（生物化学）分析
6. Analyses of gonococcal lipopolysaccharide in whole-cell lysates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis: stable association of lipopolysaccharide with the major outer membrane protein (protein I) of Neisseria gonorrhoeae. [O] . P J Hitchcock 1984

机译：通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析全细胞裂解液中的淋球菌脂多糖：脂多糖与淋病奈瑟氏球菌的主要外膜蛋白（蛋白I）稳定缔合。
7. Analyses of gonococcal lipopolysaccharide in whole-cell lysates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis: stable association of lipopolysaccharide with the major outer membrane protein (protein I) of Neisseria gonorrhoeae [O] . P J Hitchcock 1984

机译：十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳淋巴结酸碱酸酐酸淋巴瘤分析：脂多糖与奈瑟氏淋巴膜主要外膜蛋白（蛋白I）的稳定结合
8. Variable Expression of Membrane Proteins among Murine Lymphoblastoid Tumors as Seen with Sodium Dodecyl Sulfate-Polyacrylamide Gradient Gel Electophoresis of (35S) Methionine-Labeled Cell Membranes [R] . Malenka, D. J., Rogan, K. M., Howe, R. C., 1979

机译：（35s）甲硫氨酸标记细胞膜十二烷基硫酸钠 - 聚丙烯酰胺梯度凝胶电泳中小鼠淋巴母细胞瘤中膜蛋白的可变表达

Requirements for the application of protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis and randomly amplified polymorphic DNA analyses to product speciation

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