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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >DETERMINATION OF CARBOHYDRATES AS THEIR DANSYLHYDRAZINE DERIVATIVES BY CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION
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DETERMINATION OF CARBOHYDRATES AS THEIR DANSYLHYDRAZINE DERIVATIVES BY CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION

机译：毛细管电泳-激光荧光检测法测定二氢嘧啶衍生物中的碳水化合物

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A mixture of mono- and disaccharides was derivatized with dansylhydrazine in a relatively fast chemical reaction (15 min) which is selective toward aldehydes and ketones. The products of the derivatization reaction were then separated by capillary electrophoresis (CE) using laser-induced fluorescence (LIF) as the detection mode. The smallest amount of carbohydrate derivatized and determined by CE/LIF was 10 pmol contained in 100 mu L of the final reaction mixture. The CE/LIF method provided a linear response for the carbohydrates tested (over 2-3 orders of magnitude), with a limit of detection of 100 amol. Separation efficiencies over 250 000 theoretical plates were obtained. The procedure was used to analyze a tear fluid sample to demonstrate the applicability of the method for the glucose determination in small volumes of biological samples. [References: 29]
机译：单糖和二糖的混合物用丹酰肼在相对较快的化学反应（15分钟）中衍生化，该化学反应对醛和酮具有选择性。然后，使用激光诱导的荧光（LIF）作为检测模式，通过毛细管电泳（CE）分离衍生化反应的产物。通过CE / LIF衍生和测定的碳水化合物的最小量为100 pL最终反应混合物中所含的10 pmol。 CE / LIF方法对测试的碳水化合物（超过2-3个数量级）提供了线性响应，检出限为100 amol。分离效率超过250000个理论塔板。该程序用于分析眼泪液样品，以证明该方法在少量生物样品中测定葡萄糖的适用性。 [参考：29]

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