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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Effect of staining reagent on peptide mass fingerprinting from in-gel trypsin digestions: A comparison of SyproRuby (TM) and DeepPurple (TM)
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Effect of staining reagent on peptide mass fingerprinting from in-gel trypsin digestions: A comparison of SyproRuby (TM) and DeepPurple (TM)

机译：染色剂对凝胶内胰蛋白酶消化肽质量指纹图谱的影响：SyproRuby（TM）和DeepPurple（TM）的比较

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摘要

As the new fluorescent stains such as SyproRuby (TM) and DeepPurple (TM) are getting widespread recognition for proteome analyses by the traditional 2-D gel method, it becomes important to test the feasibility of these stains with respect to staining reproducibility, protein quantitation, and compatibility of the stain with downstream MS. The binding of epicocconone, active ingredient of DeepPurple (TM), to one of the primary cleavage sites of trypsin (lysine residue) raises the possibility of incomplete cleavage and interference with PMF However, the current study tests and concludes that the DeepPurple (TM) stain can result in increased peptide recovery compared to SyproRuby (TM) stain and can improve MS-based identification of lower intensity proteins spots.
机译：随着新的荧光染料，例如SyproRuby（TM）和DeepPurple（TM），通过传统的2-D凝胶法在蛋白质组分析中得到广泛认可，测试这些染料在染色再现性，蛋白质定量方面的可行性变得非常重要。以及污渍与下游MS的相容性。 Epicocconone（DeepPurple（TM）的活性成分）与胰蛋白酶（赖氨酸残基）的一个主要裂解位点的结合增加了不完全裂解和干扰PMF的可能性。然而，当前的研究测试并得出结论，DeepPurple（TM）与SyproRuby（TM）染色相比，该染色可以提高肽的回收率，并且可以改善基于MS的低强度蛋白斑点的鉴定。

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