Studying protein-drug interaction by microfluidic chip affinity capillary electrophoresis with indirect laser-induced fluorescence detection

Liu XJ; Liu X; Liang AY; Shen Z; Zhang Y; Dai ZP; Xiong BH; Lin BC

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Studying protein-drug interaction by microfluidic chip affinity capillary electrophoresis with indirect laser-induced fluorescence detection

机译：通过微流控芯片亲和毛细管电泳和间接激光诱导的荧光检测研究蛋白质-药物相互作用

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We developed a microfluidic chip-affinity CE method based on indirect LIF detection to study protein-drug interactions. The interaction between heparin and BSA was quantitatively studied, as a model system. In our method, sodium fluorescein was chosen as background, and redistilled water as marker to monitor EOF The electrophoretic mobility changes of BSA were measured, with various concentrations of heparin added to the running buffer. Each run was completed within 80 s. The binding constant was determined to be (1.24 +/- 0.05) x 10(3) M-1, which was in good agreement with that reported in the literature.

机译：我们开发了一种基于间接LIF检测的微流控芯片亲和CE方法，以研究蛋白质-药物相互作用。作为模型系统，对肝素和BSA之间的相互作用进行了定量研究。在我们的方法中，以荧光素钠为背景，并以重蒸馏水作为EOF的监测剂。在运行缓冲液中加入各种浓度的肝素，测定BSA的电泳迁移率变化。每次运行均在80秒内完成。测定的结合常数为（1.24 +/- 0.05）×10（3）M-1，这与文献报道的一致。

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《Electrophoresis: The Official Journal of the International Electrophoresis Society》 |2006年第15期|共4页
作者
Liu XJ; Liu X; Liang AY; Shen Z; Zhang Y; Dai ZP; Xiong BH; Lin BC;
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正文语种 eng
中图分类化学;
关键词
binding constant; indirect LIF; microfluidic chip-affinity CE; FIBROBLAST-GROWTH-FACTOR; FRONTAL ANALYSIS; HOMOGENEOUS IMMUNOASSAY; SERUM-ALBUMIN; BINDING; HEPARIN; MICROCHIP; PLASMA; TNT;

机译：结合常数;间接LIF;微流控芯片亲和性CE;成纤维细胞生长因子;纤维蛋白分析;均质免疫分析;血清白蛋白;结合;肝素;微芯片;血浆;TNT;

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专利

1. Studying protein-drug interaction by microfluidic chip affinity capillary electrophoresis with indirect laser-induced fluorescence detection [J] . Liu XJ, Liu X, Liang AY, Electrophoresis: The Official Journal of the International Electrophoresis Society . 2006,第15期

机译：通过微流控芯片亲和毛细管电泳和间接激光诱导的荧光检测研究蛋白质-药物相互作用
2. Simultaneous Detection of Yersinia enterocolitica and Listeria monocytogenes in Foodstuffs by Capillary Electrophoresis and Microchip Capillary Electrophoresis Laser-Induced Fluorescence Detector [J] . Li Yongru, Su Hongwei, Lan Yajia Journal of AOAC International . 2018,第6期

机译：用毛细管电泳和微芯片毛细管电泳激光诱导荧光检测器同时检测食品中的yersinia Enterocolitea和Histeria单核细胞增生
3. Characterization of dissolved organic carbon at low levels in environmental waters by microfluidic-chip-based capillary gel electrophoresis with a laser-induced fluorescence detector [J] . Shuanglong Shen, Yan Li, Shin-ichi Wakida Environmental Monitoring and Assessment . 2010,第1a4期

机译：通过基于微流控芯片的毛细管凝胶电泳和激光诱导荧光检测器表征环境水中的低水平溶解有机碳
4. Study of Laser-induced fluorescence detection for Capillary Electrophoresis chip based on optical fiber coupled [C] . Yan Weiping, Mu Xiaomin, Yan Ping, International Symposium on Advanced Optical Manufacturing and Testing Technologies; 20051102-05; Xian(CN) . 2006

机译：基于光纤耦合的毛细管电泳芯片激光诱导荧光检测的研究
5. The use of covalent and noncovalent labeling schemes for the study of bioaffinity interactions via capillary electrophoresis with near-infrared laser-induced fluorescence detection. [D] . Sowell, John Day. 2003

机译：使用共价和非共价标记方案通过毛细管电泳与近红外激光诱导的荧光检测研究生物亲和力相互作用。
6. Indirect detection of superoxide in RAW 264.7 macrophage cells using microchip electrophoresis coupled to laser-induced fluorescence [O] . Richard P. S. de Campos, Joseph M. Siegel, Claudia G. Fresta, -1

机译：微芯片电泳结合激光诱导的荧光间接检测RAW 264.7巨噬细胞中的超氧化物
7. Interplay of Binding Stoichiometry and Recognition Specificity for the Interaction of MBD2b Protein and Methylated DNA Revealed by Affinity Capillary Electrophoresis Coupled with Laser-Induced Fluorescence Analysis [O] . Zou Dandan, Zhang Dapeng, Liu Shengquan, 2014

机译：亲和毛细管电泳结合激光诱导荧光分析揭示的MBD2b蛋白与甲基化DNA相互作用的化学计量学和识别特异性之间的相互作用。
8. Applications of capillary electrophoresis and laser-induced fluorescence detection to the analysis of trace species: From single cells to single molecules [R] . Qifeng, X. 1995

机译：毛细管电泳和激光诱导荧光检测在痕量物种分析中的应用：从单细胞到单个分子

Studying protein-drug interaction by microfluidic chip affinity capillary electrophoresis with indirect laser-induced fluorescence detection

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