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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Determination of egg white lysozyme by on-line coupled capillary isotachophoresis with capillary zone electrophoresis
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Determination of egg white lysozyme by on-line coupled capillary isotachophoresis with capillary zone electrophoresis

机译:在线耦合毛细管等速电泳-毛细管区带电泳法测定蛋清溶菌酶

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摘要

An on-line coupled capillary isotachophoresis - capillary zone electrophoresis method for the determination of lysozyme in selected food products is described. The optimized electrolyte system consisted of 10 mM NH4OH + 20 mM acetic acid (leading electrolyte), 5 mM F,epsilon-aminocaproic acid + 5 mM acetic acid (terminating electrolyte), and 20 mm F-aminocaproic acid + 5 mm acetic acid + 0.1 % m/v hydroxypropylmethylcellulose (background electrolyte). A clear separation of lysozyme from other components of acidic sample extract was achieved within 15 min. Method characteristics, i.e., linearity (0-50 mug/mL), accuracy (recovery 96 +/- 5%), intra-assay (3.8%), quantification limit (1 mug/ml), and detection limit (0.25 mug/mL) were determined. Low laboriousness, sufficient sensitivity and low running costs are important attributes of this method. The developed method is suitable for the quantification of the egg content in egg pasta. [References: 10]
机译:描述了一种在线耦合毛细管等速电泳-毛细管区带电泳法,用于测定所选食品中的溶菌酶。优化的电解质系统由10 mM NH4OH + 20 mM乙酸(主要电解质),5 mM F,ε-氨基己酸+ 5 mM乙酸(终止电解质)和20 mm F-氨基己酸+ 5 mm乙酸+组成0.1%m / v羟丙基甲基纤维素(背景电解质)。 15分钟内即可将溶菌酶与酸性样品提取物的其他组分彻底分离。方法特性,例如线性(0-50杯/ mL),准确性(回收率96 +/- 5%),测定内(3.8%),定量限(1杯/ ml)和检测限(0.25杯/ mL)测定)。低劳动强度,足够的灵敏度和较低的运行成本是该方法的重要属性。所开发的方法适用于定量鸡蛋面食中的鸡蛋含量。 [参考:10]

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