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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Combination of 768-well microplate array diagonal gel electrophoresis with duplex PCR dia of X and Y chromosome markers for quality control of epidemiological DNA banks
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Combination of 768-well microplate array diagonal gel electrophoresis with duplex PCR dia of X and Y chromosome markers for quality control of epidemiological DNA banks

机译：768孔微孔板阵列对角线凝胶电泳与X和Y染色体标记的双向PCR dia结合用于流行病学DNA库的质量控制

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摘要

Large DNA banks for human epidemiological studies have become an increasingly important research tool. The power of genotype-phenotype studies is dependent both on the quality of phenotyping and of genotyping and of correct linking of phenotypes to genotypes. Samples must be tracked through numerous steps between subject or patient and post-genotypic data. Only one phenotype, sex, has a perfect and binary correlation with genotype. In mixed sex studies, it may be advantageous for purposes of quality control to keep sexes mixed during the steps from acquisition to DNA bank, in order to be able to check later for sample swaps. We have designed a duplex PCR combining an amplicon from MAOA marking the X chromosome and an amplicon from DDX3Y marking the Y chromosome. We combined this with a simple economical palmtop sized 768-well microplate compatible electrophoresis system developed in-house for examination of duplex PCR products. We applied this quality control test in the validation of two DNA banks.
机译：用于人类流行病学研究的大型DNA库已经成为越来越重要的研究工具。基因型-表型研究的能力既取决于表型和基因型的质量，也取决于表型与基因型的正确连接。必须在受试者或患者与基因型后数据之间的许多步骤中跟踪样品。只有一种表型，性别与基因型具有完美的二元相关性。在混合性研究中，出于质量控制的目的，在从采集到DNA库的步骤中保持性别混合可能是有利的，以便以后可以检查样品交换。我们设计了一种双重PCR，将标记为X染色体的MAOA扩增子和标记Y染色体的DDX3Y扩增子结合在一起。我们将其与内部开发的简单经济的掌上型768孔微孔板兼容电泳系统结合在一起，用于检测双链PCR产品。我们在两个DNA库的验证中应用了此质量控制测试。

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