STUDIES ON THE PROCEDURE FOR THE CONSTRUCTION OF CELLULAR PROTEIN DATABASES EMPLOYING MICRO TWO-DIMENSIONAL ELECTROPHORESIS - AN HL-60 PROTEIN DATABASE

Manabe T.; Kawai M.; Yamamoto H.

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STUDIES ON THE PROCEDURE FOR THE CONSTRUCTION OF CELLULAR PROTEIN DATABASES EMPLOYING MICRO TWO-DIMENSIONAL ELECTROPHORESIS - AN HL-60 PROTEIN DATABASE

机译：二维电泳的细胞蛋白数据库构建过程的研究-HL-60蛋白数据库

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The procedure to construct a cellular protein database employing a micro two-dimensional electrophoresis (2-DE) technique was studied. Protein information on human cultured leukemic cell HL-60 was collected as an HL-60 protein database, which consists of a 2-DE polypeptide map of 670 recognized spots with spot numbering and information on isoelectric point (pI), molecular mass, and percent integrated density. The procedure employed in this report will be applicable for the comparative studies of cellular proteins and will help to construct comprehensive cellular protein databases. [References: 50]

机译：研究了使用微二维电泳（2-DE）技术构建细胞蛋白质数据库的过程。收集人类培养的白血病细胞HL-60上的蛋白质信息作为HL-60蛋白质数据库，该数据库由670个已识别斑点的2-DE多肽图组成，具有斑点编号以及等电点（pI），分子量和百分数的信息综合密度。本报告中使用的程序将适用于细胞蛋白质的比较研究，并将有助于构建全面的细胞蛋白质数据库。 [参考：50]

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《Electrophoresis: The Official Journal of the International Electrophoresis Society》 |1995年第3期|共16页
作者
Manabe T.; Kawai M.; Yamamoto H.;
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正文语种 eng
中图分类化学;
关键词
Cellular proteins; Protein database; Two-dimensional polyacrylamide gel electrophoresis; Micro two-dimensional electrophoresis; Hl-60 proteins; 2-dimensional gel database; Human-plasma-proteins; Human epidermal-keratinocytes; Amino-acid-sequences; Amnion cells ama; Cerebrospinal-fluid; Escherichia-coli; Comprehensive databases; Polyacrylamide gels; Identification;

机译：细胞蛋白;蛋白质数据库;二维聚丙烯酰胺凝胶电泳;微二维电泳;Hl-60蛋白;二维凝胶数据库;人血浆蛋白;人表皮角质形成细胞;氨基酸序列;羊膜细胞;脑脊液;大肠埃希菌;综合数据库;聚丙烯酰胺凝胶;鉴定;

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1. 多种化疗药对凋亡抑制蛋白Survivin在HL-60细胞中表达影响的研究 [J] . 吴耀辉, 邹萍, 张敏, 中德临床肿瘤学杂志（英文版） . 2006,第003期
2. STUDIES ON THE PROCEDURE FOR THE CONSTRUCTION OF CELLULAR PROTEIN DATABASES EMPLOYING MICRO TWO-DIMENSIONAL ELECTROPHORESIS - AN HL-60 PROTEIN DATABASE [J] . Manabe T., Kawai M., Yamamoto H. Electrophoresis: The Official Journal of the International Electrophoresis Society . 1995,第3期

机译：二维电泳的细胞蛋白数据库构建过程的研究-HL-60蛋白数据库
3. An iterative calibration method with prediction of post-translational modifications for the construction of a two-dimensional electrophoresis database of mouse mammary gland proteins [J] . Sevil Aksu, Christian Scheler, Nicole Focks, Proteomics . 2002,第10期

机译：预测翻译后修饰的迭代校准方法，用于构建小鼠乳腺蛋白的二维电泳数据库
4. CONSTRUCTION OF HSC-2DPAGE - A TWO-DIMENSIONAL GEL ELECTROPHORESIS DATABASE OF HEART PROTEINS [J] . Evans G., Corbett JM., Dunn MJ., Electrophoresis: The Official Journal of the International Electrophoresis Society . 1997,第3a4期

机译：HSC-2DPAGE的构建-二维凝胶蛋白电泳蛋白质数据库
5. Protein database construction for microblial community protemics and ipmpacts on peptide and protein assignments, confidence, quantitation and differential analysis [C] . David A. C. Beck, Erik L. Hendrickson, Tiansong WANG, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012

机译：蛋白质数据库结构，用于微丑体群落的纯度和IPMPacts对肽和蛋白质分配，置信度，定量和差异分析
6. Characterization of community-acquired methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis, multilocus sequence typing, and Staphylococcal protein A sequencing: Establishing a strain typing database. [D] . Roberts, Jill Carolyne. 2006

机译：通过脉冲场凝胶电泳，多基因座序列分型和葡萄球菌蛋白A测序表征社区获得的耐甲氧西林金黄色葡萄球菌：建立菌株分型数据库。
7. Degradation of cellular proteins during poliovirus infection: studies by two-dimensional gel electrophoresis. [O] . A Urzainqui, L Carrasco 1989

机译：脊髓灰质炎病毒感染过程中细胞蛋白质的降解：通过二维凝胶电泳进行研究。
8. Separation of Total Nuclear Proteins by an Improved Two-Dimensional Polyacrylamide Gel Electrophoresis : The Method and Applications to the Study of Induced Differentiation of HL-60 Cells (Commemoration Issue Dedicated to Professor Hiroshi Ibagaki, Professor Michio Kurata, Professor Ryozo Kitamura, On the Occasion of Their Retirments) [O] . Hazama Masatoshi, Shimokawa Hisatoshi, Shimamoto Nobuo, 1989

机译：改进的二维聚丙烯酰胺凝胶电泳分离总核蛋白：HL-60细胞诱导分化研究的方法和应用（纪念刊物由Ibagaki教授，仓田道夫教授，北村良三教授在场合发表他们的退休生活
9. Procedures for two-dimensional electrophoresis of proteins [R] . Tollaksen, S. L., Giometti, C. S. 1996

机译：蛋白质双向电泳的程序

STUDIES ON THE PROCEDURE FOR THE CONSTRUCTION OF CELLULAR PROTEIN DATABASES EMPLOYING MICRO TWO-DIMENSIONAL ELECTROPHORESIS - AN HL-60 PROTEIN DATABASE

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