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首页> 外文期刊>Bulletin of experimental biology and medicine >Optimized Protocol for Isolation of Multipotent Mesenchymal Stromal Cells from Human Umbilical Cord
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Optimized Protocol for Isolation of Multipotent Mesenchymal Stromal Cells from Human Umbilical Cord

机译：从人脐带中分离多能间质基质细胞的优化方案

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摘要

Extraembryonic tissues, in particular, umbilical cord stroma are promising sources of multipotent mesenchymal stromal cells for regenerative medicine. In recent years, methods for isolation of mesenchymal stromal cells from different compartments of the umbilical cords based on enzymatic disaggregation of the tissue or on tissue explants have been proposed. Here we propose a protocol of isolation of multipotent mesenchymal stromal cells from the whole umbilical cord that combines the advantages of each approach and ensures sufficient cell yield for further experimental and clinical applications. A combination of short-term incubation of tissue fragments on cold collagenase solution followed by their culturing in the form of explants significantly increased the yield of cells with high proliferative activity, typical pluripotent mesenchymal stromal cell phenotype, and preserved differentiation capacity.
机译：胚外组织，特别是脐带基质是用于再生医学的多能间充质基质细胞的有前途的来源。近年来，已经提出了基于组织的酶分解或组织外植体从脐带的不同隔室分离间充质基质细胞的方法。在这里，我们提出了一种从整个脐带中分离多能间充质基质细胞的方案，该方案结合了每种方法的优点，并确保了足够的细胞产量以用于进一步的实验和临床应用。组织片段在冷胶原酶溶液上短期孵育，然后以外植体形式培养的组合，可显着提高具有高增殖活性，典型多能间质基质细胞表型和保留分化能力的细胞的产量。

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