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首页> 外文期刊>EMBO reports >Acetylation regulates monopolar attachment at multiple levels during meiosis i in fission yeast
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Acetylation regulates monopolar attachment at multiple levels during meiosis i in fission yeast

机译:乙酰化在裂变酵母减数分裂过程中在多个水平上调节单极附着

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摘要

In fission yeast, meiotic mono-orientation of sister kinetochores is established by cohesion at the core centromere, which is established by a meiotic cohesin complex and the kinetochore protein Moa1. The cohesin subunit Psm3 is acetylated by Eso1 and deacetylated by Clr6. We show that in meiosis, Eso1 is required for establishing core centromere cohesion during S phase, whereas Moa1 is required for maintaining this cohesion after S phase. The clr6-1 mutation suppresses the mono-orientation defect of moa1δ " cells, although the Clr6 target for this suppression is not Psm3. Thus, several acetylations are crucial for establishing and maintaining core centromere cohesion.
机译:在裂变酵母中,姊妹运动体的减数分裂单向是通过核心着丝粒的凝聚而建立的,核心着丝粒是由减数分裂粘附素复合物和动粒蛋白Moa1建立的。粘附素亚基Psm3被Eso1乙酰化并被Clr6脱乙酰化。我们表明,在减数分裂中,Eso1是建立S期核心核心着丝粒内聚力所必需的,而Moa1是维持S期后这种内聚力所必需的。尽管抑制的目标不是Psm3,但是clr6-1突变抑制了moa1δ“细胞的单向缺陷。因此,几种乙酰化对于建立和维持核心着丝粒的凝聚力至关重要。

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