...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Endocrinology >Hormonal modulation of amino acid neurotransmitter metabolism in the arcuate nucleus of the adult female rat: a novel action of estradiol.
【24h】

Hormonal modulation of amino acid neurotransmitter metabolism in the arcuate nucleus of the adult female rat: a novel action of estradiol.

机译：成年雌性大鼠弓状核中氨基酸神经递质代谢的激素调节：雌二醇的新作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Morphological plasticity in response to estradiol is a hallmark of astrocytes in the arcuate nucleus. The functional consequences of these morphological changes have remained relatively unexplored. Here we report that in the arcuate nucleus estradiol significantly increased the protein levels of the two enzymes in the glutamate-glutamine cycle, glutamine synthetase and glutaminase. We further demonstrate that these estradiol-mediated changes in the enzyme protein levels may underlie functional changes in neurotransmitter availability as: 1) total glutamate concentration in the arcuate nucleus was significantly increased and 2) microdialysis revealed a significant increase in extracellular glutamate levels after a synaptic challenge in the presence of estradiol. These data implicate the glutamate-glutamine cycle in the generation and/or maintenance of glutamate and suggest that the difference in extracellular glutamate between estradiol- and oil-treated animals may be related to an increased efficiency of the cycle enzymes. In vivo enzyme activity assays revealed that the estradiol mediated increase in glutamate-glutamine cycle enzymes in the arcuate nucleus led to an increase in gamma-aminobutyric acid and is likely not related to the increase in extracellular glutamate. Thus, we have observed two-independent effects of estradiol on amino acid neurotransmission in the arcuate nucleus. These data suggest a possible functional consequence of the well-established changes in glial morphology that occur in the arcuate nucleus in the presence of estradiol and suggest the importance of neuronal-glial cooperation in the regulation of hypothalamic functions such as food intake and body weight.
机译：响应雌二醇的形态可塑性是弓形核中星形胶质细胞的标志。这些形态变化的功能后果尚未得到充分研究。在这里，我们报告说，在弓形核中，雌二醇显着增加了谷氨酸-谷氨酰胺循环中两种酶，谷氨酰胺合成酶和谷氨酰胺酶的蛋白质水平。我们进一步证明，这些雌二醇介导的酶蛋白水平变化可能是神经递质可用性的功能变化的基础，因为：1）弓状核中的总谷氨酸浓度显着增加，2）微透析显示突触后细胞外谷氨酸水平显着增加。在雌二醇存在下挑战。这些数据暗示谷氨酸-谷氨酰胺循环在谷氨酸的产生和/或维持中，并且表明雌二醇和油处理的动物之间的细胞外谷氨酸的差异可能与循环酶效率的提高有关。体内酶活性分析表明，雌二醇介导的弓形核中谷氨酸-谷氨酰胺循环酶的增加导致了γ-氨基丁酸的增加，并且可能与细胞外谷氨酸的增加无关。因此，我们已经观察到雌二醇对弓形核中氨基酸神经传递的两个独立影响。这些数据表明在雌二醇存在下弓形核中神经胶质形态的确定变化可能产生的功能后果，并提示神经元-神经胶质合作在调节下丘脑功能（如食物摄入和体重）中的重要性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号