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首页> 外文期刊>Endocrinology >The corpuscles of Stannius, calcium-sensing receptor, and stanniocalcin: responses to calcimimetics and physiological challenges.
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The corpuscles of Stannius, calcium-sensing receptor, and stanniocalcin: responses to calcimimetics and physiological challenges.

机译：斯坦尼斯小球，钙敏感受体和斯坦诺钙素的小球：对拟钙剂和生理挑战的反应。

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This study has examined whether the calcium-sensing receptor (CaSR) plays a role in control of stanniocalcin-1 (STC-1), the dominant calcium regulatory hormone of fish, comparable with that demonstrated for CaSR in the mediation of ionized calcium regulation of PTH secretion in mammals. In a previous study, we have cloned flounder STC-1 from the corpuscles of Stannius (CS). Here, we report the cloning and characterization of the CS CaSR, and the in vivo responses of this system to altered salinity, EGTA induced hypocalcemia, and calcimimetic administration. Quantitative PCR analysis demonstrated, for the first time, that the CS are major sites of CaSR expression in flounder. Immunoblot analysis of CS proteins with CaSR-specific antibodies revealed a broad band of approximately 215-300 kDa under nonreducing conditions, and bands of approximately 215-300 kDa and approximately 120-150 kDa under reducing conditions. There were no differences in CS CaSR mRNA expression or plasma STC-1 levels between seawater and freshwater (FW)-adapted fish, although CS STC-1 mRNA expression was lower in FW animals. Immunoblots showed that glycosylated monomeric forms of the CaSR migrated at a lower molecular mass in CS samples from FW animals. The ip administration of EGTA rapidly induced hypocalcemia, and a concomitant lowering of plasma STC-1. Calcimimetic administration (1 mg/kg R-568) rapidly increased plasma STC-1 levels, and reduced plasma concentrations of calcium, phosphate, and magnesium when compared with S-568-treated controls. Together, these findings support an evolutionary conserved role for the CaSR in the endocrine regulation of calcium before the appearance of parathyroid glands in tetrapods.
机译：这项研究检查了钙敏感受体（CaSR）是否在控制鱼类主要钙调节激素stanniocalcin-1（STC-1）中发挥作用，与CaSR在介导的钙离子调节中的作用相当。哺乳动物中PTH的分泌。在先前的研究中，我们从斯坦尼乌斯（CS）小球克隆了比目鱼STC-1。在这里，我们报告了CS CaSR的克隆和表征，以及该系统对盐度变化，EGTA引起的低血钙和拟钙剂给药的体内反应。定量PCR分析首次证明，CS是比目鱼CaSR表达的主要位点。用CaSR特异性抗体对CS蛋白进行的免疫印迹分析显示，在非还原条件下，约215-300 kDa的条带宽，在还原条件下，约215-300 kDa的条带和120-150 kDa的条带。海水和淡水（FW）适应鱼之间CS CaSR mRNA表达或血浆STC-1水平没有差异，尽管FW动物中CS STC-1 mRNA表达较低。免疫印迹显示，在FW动物的CS样品中，CaSR的糖基化单体形式以较低的分子质量迁移。 ipta的ip给药迅速引起低血钙症，并伴随血浆STC-1降低。与S-568处理的对照组相比，拟钙剂给药（1 mg / kg R-568）迅速提高了血浆STC-1水平，并降低了血浆钙，磷酸盐和镁的浓度。总之，这些发现支持在四足动物的甲状旁腺出现之前CaSR在钙的内分泌调节中的进化保守作用。

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