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首页> 外文期刊>Electroanalysis >Construction and Application of Flow Enzymatic Biosensor Based of Silver Solid Amalgam Electrode for Determination of Sarcosine
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Construction and Application of Flow Enzymatic Biosensor Based of Silver Solid Amalgam Electrode for Determination of Sarcosine

机译：银固体汞齐电极流动酶生物传感器测定肌氨酸的应用

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摘要

In this paper, the flow amperometric enzymatic biosensor based on polished silver solid amalgam electrode for determination of sarcosine in model sample under flow injection analysis conditions is presented. The biosensor works on principle of electrochemical detection of oxygen decrease during enzymatic reaction which is directly proportional to the concentration of sarcosine in sample. The whole preparation process takes about 3 h. The RSD of repeatability of 10 consecutive measurements is 1.6% (c(sarcosine)= 1.0 x 10(-4) mol dm(-3)). Under optimal conditions the calibration dependence was linear in the range 7.5 x 10(-6)-5.0 x 10(-4) mol dm(-3) and limit of detection was 2.0 x 10(-6) mol dm(-3).
机译：本文提出了一种基于抛光银固体汞合金电极的流量安培酶生物传感器，用于在流动注射分析条件下测定模型样品中的肌氨酸。该生物传感器的工作原理是通过电化学检测酶促反应过程中氧的减少，氧的减少与样品中肌氨酸的浓度成正比。整个准备过程大约需要3个小时。 10次​​连续测量的重复性RSD为1.6％（c（肌氨酸）= 1.0 x 10（-4）mol dm（-3））。在最佳条件下，校准依赖性在7.5 x 10（-6）-5.0 x 10（-4）mol dm（-3）范围内呈线性，检出限为2.0 x 10（-6）mol dm（-3） 。

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