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首页> 外文期刊>Electroanalysis >Evaluation of heat resistance of microorganisms by an amperometric measurement of the microbial substrate-oxidizing activity using a whole-cell modified electrode
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Evaluation of heat resistance of microorganisms by an amperometric measurement of the microbial substrate-oxidizing activity using a whole-cell modified electrode

机译：使用全细胞修饰电极通过安培法测量微生物底物的氧化活性来评估微生物的耐热性

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A mediated bioelectrocatalysis using a bacterial cell-modified electrode was applied to the evaluation of heat resistance of Escherichia coli and Lactobacillus plantarum. The first derivative of the time course of the bioelectrocatalysis current for the bacterial D-glucose oxidation had a maximum d I/dt(max) at about 10 to 60 s after the addition of D-glucose to the solution. The dI/dt(max) value decreased in different manners depending on the conditions of heat treatment of the bacterial cells prior to their immobilization on electrode. The decreasing tendency in the dI/dt(max) value was similar to that in the colony formation unit measured by a conventional colony counting method. The dI/dt(max) method which takes only 2 h, is much more time saving compared with the conventional method taking 24 h incubation for E. coli and 72 h incubation for L. plantarum. In some cases, however, there was a difference in the magnitude of relative decrease between the two methods, which could be attributed to the appearance of injured cells that were active in the dI/dt(max) measurement but not in the colony formation. [References: 13]
机译：使用细菌细胞修饰的电极介导的生物电催化被应用于评估大肠杆菌和植物乳杆菌的耐热性。在向溶液中添加D-葡萄糖后，细菌D-葡萄糖氧化的生物电催化电流的时间过程的一阶导数在约10至60 s处具有最大d I / dt（max）。 dI / dt（max）值以不同的方式降低，具体取决于细菌细胞固定在电极上之前的热处理条件。 dI / dt（max）值的降低趋势类似于通过常规菌落计数方法测得的菌落形成单位中的趋势。 dI / dt（max）方法仅需2小时，与传统方法（对于大肠杆菌进行24小时孵育和对植物乳杆菌进行72小时孵育）相比，可以节省更多时间。但是，在某些情况下，两种方法的相对降低幅度存在差异，这可能归因于在dI / dt（max）测量中活跃但在集落形成中不活跃的受损细胞的出现。 [参考：13]

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