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Mitochondria-based amperometric biosensor for the determination of L-glutamic acid

机译:基于线粒体的安培生物传感器测定L-谷氨酸

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A mitochondria based amperometric biosensor was developed for the determination of L-glutamic acid. The biosensor was characterized using hexacyanoferrate(III) as the electrochemical mediator. The electrochemical behavior of the redox couple hexacyanoferrate(II)/(III) was improved by incorporating octadecylamine into the enzyme modified carbon paste. L-Glutamic acid was detected at +0.35 V Linearity was observed in the range 0.4-10 mM of L-glutamic acid (sensitivity 0.189 muA/mM, correlation coefficient 0.997) and in the range 10-100mM (sensitivity 0.021 muA/mM. correlation coefficient 0.958). The detection limit was 0.1 mM. The electrode maintains 60% of its activity after 10 days of use. The bioelectrode was used for the determination of L-glutamate in chicken cube samples. [References: 29]
机译:基于线粒体的安培生物传感器被开发用于测定L-谷氨酸。使用六氰基高铁酸盐(III)作为电化学介质来表征生物传感器。通过将十八烷基胺掺入酶改性的碳糊中,可以改善氧化还原对六氰合铁酸酯(II)/(III)的电化学行为。在+0.35 V处检测到L-谷氨酸。在L-谷氨酸的0.4-10 mM范围(灵敏度0.189μA/ mM,相关系数0.997)和10-100mM的线性范围(灵敏度0.021μA/ mM)中观察到线性。相关系数0.958)。检测极限为0.1mM。使用10天后,电极可保持其60%的活性。该生物电极用于测定鸡块样品中的L-谷氨酸。 [参考:29]

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