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首页> 外文期刊>Electroanalysis >Development of an electroanalytical method for the quantification of zearalenone (ZEA) in maize samples
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Development of an electroanalytical method for the quantification of zearalenone (ZEA) in maize samples

机译：开发一种用于分析玉米样品中玉米赤霉烯酮（ZEA）的电分析方法

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摘要

The application of electroanalytical techniques to detect and quantify zearalenone (ZEA) mycotoxin that frequently contaminates maize and foodstuff is studied in this work. Rice and maize grains were inoculated with Fusarium fungus to obtain ZEA in artificially infected samples. The electro-oxidation of ZEA adsorbed on the surface of glassy carbon (GC) electrodes in 20% acetonitrile (ACN) + 80% 1 M HClO4 (aqueous solution) reaction medium was studied by using square-wave voltammetry (SWV). Studies were conducted to find the most favorable accumulation potential (E-acc) and accumulation time (t(acc)) to perform the ZEA preconcentration on the electrode surface. It was found that E-acc was any value in the range from 0.00 - 0.90 V and the best t(acc) was 120 s, respectively, for ZEA separated from extracting solution by TLC (ZEA(TLC)) while E-acc = 0.90 V corresponded to ZEA in non separated matrix solution (ZEA(matrix)). The ZEA quantitative determination was performed by SWV combined with the standard addition method. Linear plots were obtained from the net peak current (I-p,I-n) vs c*(ZEA) in the concentration range from 20 to 3184 ppb. Detection limit of 30 ppb at a signal to noise ratio of 3:1 was obtained. On the other hand, recovery experiments were performed on uncontaminated maize samples spiked with ZEA.
机译：在这项工作中，研究了电分析技术在检测和定量经常污染玉米和食品的玉米赤霉烯酮（ZEA）霉菌毒素中的应用。水稻和玉米籽粒接种了镰刀菌真菌，可在人工感染的样品中获得ZEA。使用方波伏安法（SWV）研究了吸附在玻璃碳电极上的ZEA在20％乙腈（ACN）+ 80％1 M HClO4（水溶液）反应介质中的电氧化。进行研究以发现最有利的累积电位（E-acc）和累积时间（t（acc）），以在电极表面进行ZEA预浓缩。发现通过TLC（ZEA（TLC））从萃取溶液中分离出的ZEA的E-acc值在0.00-0.90 V范围内，最佳t（acc）分别为120 s，而E-acc = 0.90 V对应于非分离基质溶液（ZEA（matrix））中的ZEA。 ZEA定量测定是通过SWV结合标准添加方法进行的。从浓度范围为20至3184 ppb的净峰值电流（I-p，I-n）与c *（ZEA）得出线性图。信噪比为3：1时，检测限为30 ppb。另一方面，对掺有ZEA的未污染玉米样品进行了回收实验。

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