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首页> 外文期刊>Electroanalysis >Reticulated vitreous carbon-based composite enzyme electrodes as suitable biosensors in both aqueous and predominantly nonaqueous media
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Reticulated vitreous carbon-based composite enzyme electrodes as suitable biosensors in both aqueous and predominantly nonaqueous media

机译：网状玻璃碳基复合酶电极在水性和主要是非水性介质中均是合适的生物传感器

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摘要

The construction and performance of reticulated vitreous carbon (RVC)-based composite enzyme electrodes in different aqueous and predominantly nonaqueous working media is reported. Both the mediator (ferrocene or ferrocyanide) and the enzyme peroxidase were immobilized by direct adsorption on chemically pretreated RVC cylinders. Pores of RVC were filled up with epoxy resin. The working media considered were: an aqueous phosphate solution of pH 7.4, a predominantly nonaqueous medium constituted by acetonitrile:Tris buffer (pH 7.4) mixtures, and organized media such as micellar solutions and oil-in-water emulsions. Hydrogen peroxide and 2-butanone peroxide were used as the substrates. The composite biosensors showed a good stability in ail these media. Polishing of the electrode surface everyday before working was necessary, the useful lifetime of each single electrode being of approximately 20 days. Furthermore, reproducible responses were obtained with different electrodes constructed in the same manner. Kinetic parameters V-m, K-m,K-app and k(cat)' were calculated in all cases, as well as the analytical characteristics from the corresponding calibration plots. The detection limits obtained with the RVC-epoxy resin composite enzyme electrodes are, in general, considerably better than those found in the literature for bioelectrodes constructed with epoxy resin as the binder. Finally, this composite electrode also performed well in the flow injection mode. [References: 29]
机译：据报道，在不同的水性和主要是非水性工作介质中，基于网状玻璃碳（RVC）的复合酶电极的构建和性能。介质（二茂铁或亚铁氰化物）和过氧化物酶都通过直接吸附固定在经过化学预处理的RVC圆筒上。 RVC的孔充满环氧树脂。所考虑的工作介质为：pH 7.4的磷酸盐水溶液，主要由乙腈：Tris缓冲液（pH 7.4）混合物组成的非水介质，以及有组织的介质，例如胶束溶液和水包油乳液。过氧化氢和过氧化2-丁酮用作底物。复合生物传感器在所有这些介质中均显示出良好的稳定性。必须每天在工作前对电极表面进行抛光，每个单个电极的使用寿命约为20天。此外，使用以相同方式构造的不同电极可获得可再现的响应。在所有情况下均计算了动力学参数V-m，K-m，K-app和k（cat）'，并从相应的校准图中计算了分析特性。通常，用RVC-环氧树脂复合酶电极获得的检出限明显好于文献中对于以环氧树脂作为粘合剂构建的生物电极的检出限。最后，该复合电极在流动注射模式下也表现良好。 [参考：29]

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