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首页> 外文期刊>Electroanalysis >DNA Determination In the Presence of Copper in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry at the Mercury Film Electrode
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DNA Determination In the Presence of Copper in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry at the Mercury Film Electrode

机译：汞膜电极上吸附溶出伏安法测定稀碱性电解质中铜的DNA。

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摘要

A stripping method for the determination of single-stranded DNA in presence of copper at the submicromolar concentration levels is described. The method is based on controlled adsorptive accumulation of adenine (from acid-treated DNA) at thin-film mercury electrode followed by linear scan voltammetry measurement of the surface species. Optimum experimental conditions were found to be the use of a 5.0 x IO~3 M NaOH solution, an accumulation potential of -0.40 Vand a scan rate of 200 raV s""'. The response of adenine-copper is linear over the concentration range 50-250 ppb. For an accumulation time of 15 minutes, the detection limit was found to be 4 ppb. The more convenient relation to measuring the ssDNA in presence of metals and nitrogenated bases were also investigated. The utility of the method is demonstrated by the presence of adenosine-triphosphate (ATP) and amino acids.
机译：描述了一种在亚微摩尔浓度水平下在铜存在下测定单链DNA的剥离方法。该方法基于薄膜汞电极上腺嘌呤（经酸处理的DNA）的可控吸附性积累，然后对表面物种进行线性扫描伏安法测量。发现最佳实验条件是使用5.0 x IO〜3 M NaOH溶液，-0.40 V的累积电势和200 raV s“”的扫描速率。腺嘌呤-铜的反应在50-250 ppb的浓度范围内呈线性关系。对于15分钟的累积时间，发现检测限为4 ppb。还研究了在金属和氮化碱存在下测量ssDNA的更方便的关系。该方法的实用性通过三磷酸腺苷（ATP）和氨基酸的存在来证明。

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