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首页> 外文期刊>Electroanalysis >A biosensor system for chlorophenols using chloroperoxidase and a glucose oxidase based amperometric electrode
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A biosensor system for chlorophenols using chloroperoxidase and a glucose oxidase based amperometric electrode

机译：使用基于氯过氧化物酶和葡萄糖氧化酶的安培电极的氯酚生物传感器系统

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摘要

A combined electrocatalytic approach using chloroperoxidase and a glucose oxidase electrode has been developed for the measurement of chlorophenol (CP) congeners. Chloroperoxidase possessed a capability to generate a significant or detectable amount of electroactive species from the oxidation of 4-CP, 2,3-dichlorophenol (DCP), 2,4-DCP, 2,5-DCP, 3,4-DCP, 3,5-DCP, 2,3,4-trichlorophenol (TCP), 2,3,5-TCP, 2,3,6-TCP, 2,4,5-TCP, 2,4,6-TCP, and 2,3,4,6-tetraCP. The optimal oxidation conditions were established as 33 mu M chlorophenol, 33 U/mL chloroperoxidase and 1.33 mM hydrogen peroxide for 15 min at ambient temperature. The chlorophenol oxidation products have also been characterized using UV-vis spectrophotometry and cyclic voltammetry (CV). Together with glucose oxidase immobilized on a working glassy carbon electrode (+0.45 V vs. Ag/AgCl), chlorinated oxidation products have been demonstrated as efficient mediators in a glucose oxidase/glucose system. However, the oxidation products from the enzymatic reaction with 2-CP, 3-CP, 2,6-DCP, 3,4,5-TCP, 2,3,5,6-tetraCP and pentachlorophenol were not electroactive or capable of recycling reduced glucose oxidase to its reactive state. [References: 21]
机译：已经开发了一种使用氯过氧化物酶和葡萄糖氧化酶电极的组合电催化方法，用于测量氯酚（CP）同系物。氯过氧化物酶具有通过氧化4-CP，2,3-二氯苯酚（DCP），2,4-DCP，2,5-DCP，3,4-DCP，3产生大量或可检测量的电活性物质的能力。 ，5-DCP，2,3,4-三氯苯酚（TCP），2,3,5-TCP，2,3,6-TCP，2,4,5-TCP，2,4,6-TCP和2 ，3,4,6-tetraCP。最佳氧化条件是在环境温度下15分钟建立33μM氯酚，33 U / mL氯过氧化物酶和1.33 mM过氧化氢。还使用紫外可见分光光度法和循环伏安法（CV）对氯酚氧化产物进行了表征。与固定在玻璃态碳电极上的葡萄糖氧化酶（+0.45 V对Ag / AgCl）一起，氯化氧化产物已被证明是葡萄糖氧化酶/葡萄糖系统中的有效介体。但是，与2-CP，3-CP，2,6-DCP，3,4,5-TCP，2,3,5,6-tetraCP和五氯苯酚的酶促反应产生的氧化产物不具有电活性或能够回收利用还原葡萄糖氧化酶至其反应状态。 [参考：21]

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