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首页> 外文期刊>Electroanalysis >Effect of voltage on the topography of alkanethiol and poly(amidoamine) dendrimer layers with immobilized glucose oxidase. An atomic force microscopy study
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Effect of voltage on the topography of alkanethiol and poly(amidoamine) dendrimer layers with immobilized glucose oxidase. An atomic force microscopy study

机译：电压对固定化葡萄糖氧化酶的链烷硫醇和聚（酰胺基胺）树状聚合物层形貌的影响。原子力显微镜研究

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摘要

We applied atomic force microscopy (AFM) to study the surface topography of the supported thin films of various composition: hexadecanethiol (HDT), poly(amidoamine) dendrimers (PAMAM) of first generation (G1), mixture of HDT + G1, glucose oxidase (GOX) and HDT + G1 + GOX layers. The AFM image of dendrimers; in air revealed dome-shaped, randomly distributed aggregates of the size from 100 to 200 nm. GOX adsorbed on the gold support also formed aggregates of the diameter between 700-900 nm and height up to 36 nm. The AFM images of the HDT + G1 + GOX layer in air revealed smooth surfaces with irregular pinholes, although these were corrugated in the buffer. Application of the voltage of + 670 mV to the layers during one hour resulted in significant increase of the surface root means square roughness (R-rms). The electrical properties of G1 layers studied by cyclic voltammetry do not differ substantially from bare gold electrode, while HDT+G1 mixed layers revealed intermediate electrical properties between HDT and G1. Immobilization of GOX onto the HDT+G1 resulted further decrease of the layer permeability.
机译：我们应用原子力显微镜（AFM）研究了各种组成的支撑薄膜的表面形貌：十六烷硫醇（HDT），第一代（G1）的聚（胺基胺）树状大分子（PAMAM），HDT + G1的混合物，葡萄糖氧化酶（GOX）和HDT + G1 + GOX图层。树枝状聚合物的AFM图像;在空气中显示出圆顶状的，随机分布的聚集体，大小从100到200 nm。吸附在金载体上的GOX还形成了直径在700-900 nm之间且高度高达36 nm的聚集体。空气中HDT + G1 + GOX层的AFM图像显示出具有不规则针孔的光滑表面，尽管它们在缓冲液中呈波纹状。一小时内在各层上施加+ 670 mV的电压会导致表面均方根粗糙度（R-rms）显着增加。通过循环伏安法研究的G1层的电学性质与裸金电极没有实质性差异，而HDT + G1混合层显示出HDT和G1之间的中间电学性质。 GOX固定在HDT + G1上导致层渗透率进一步降低。

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