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首页> 外文期刊>Electroanalysis >Study on direct Electrochemistry of glucose oxidase stabilized by cross-linking and immobilized in silica nanoparticle films
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Study on direct Electrochemistry of glucose oxidase stabilized by cross-linking and immobilized in silica nanoparticle films

机译：交联稳定化并固定在二氧化硅纳米颗粒薄膜中的葡萄糖氧化酶的直接电化学研究

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摘要

Glucose oxidase (GOD) in SiO2 nanoparticle films was cross-linked by glutaraldehyde (GA), forming GOD-GA-SiO2 films on the surface of pyrolytic graphite (PG) electrodes. GOD in the GOD-GA-SiO2 films showed a pair of well defined and quasi-reversible cyclic voltammetric peaks at -0.48 V vs. SCE in pH 7.0 buffers. Scanning electron microscopy of the films demonstrated that the interaction between GOD and GA/SiO2 would make the morphology of dry GOD-GA-SiO2 films very different from that of SiO2 and GOD-SiO2 films, IR spectroscopy confirms that the GODs in the films are indeed cross-linked through GA, and GA in the films greatly improves the film stability. UV-vis and IR spectroscopy suggest that the enzyme is not denatured by cross-linking in the films. The comparative bioelectrocatalytic study with free flavin adenine dinucleotide (FAD) in SiO2 films indicates that GOD in the films retains its biocatalytic activity toward glucose oxidation. All these results show that SiO2 nanoparticle films not only provide a favorable microenvironment for the native GOD to exchange electrons directly with underlying electrodes, but also supply a matrix for stabilizing GOD when combined with GA.
机译：SiO2纳米颗粒薄膜中的葡萄糖氧化酶（GOD）通过戊二醛（GA）交联，在热解石墨（PG）电极表面上形成GOD-GA-SiO2薄膜。在pH 7.0缓冲液中，相对于SCE，GOD-GA-SiO2膜中的GOD在-0.48 V vs. SCE处显示了一对定义良好且准可逆的循环伏安峰。薄膜的扫描电子显微镜观察表明，GOD与GA / SiO2之间的相互作用会使干燥的GOD-GA-SiO2薄膜的形态与SiO2和GOD-SiO2薄膜的形态有很大不同，红外光谱证实薄膜中的GOD为确实通过GA交联，并且薄膜中的GA大大提高了薄膜的稳定性。紫外可见光谱和红外光谱表明该酶不会因薄膜中的交联而变性。在SiO2膜中使用游离黄素腺嘌呤二核苷酸（FAD）进行的比较生物电催化研究表明，膜中的GOD保留了其对葡萄糖氧化的生物催化活性。所有这些结果表明，SiO 2纳米颗粒膜不仅为天然的GOD提供了良好的微环境，使其直接与下面的电极交换电子，而且还提供了与GA结合时用于稳定GOD的基质。

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