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首页> 外文期刊>Electroanalysis >A Glucose Biosensor Based on Immobilization of Glucose Oxidase into 3D Macroporous TiO2
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A Glucose Biosensor Based on Immobilization of Glucose Oxidase into 3D Macroporous TiO2

机译：基于将葡萄糖氧化酶固定到3D大孔TiO2中的葡萄糖生物传感器

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摘要

3D macroporous TiO2 inverse opals have been derived from a sol-gel procedure using polystyrene colloidal crystals as templates. EDS and SEM showed a face-centered cubic (FCC) structure TiO2 inverse opal was obtained. Glucose oxidase (GOx) was successfully immobilized on the surface of indium-tin oxide (ITO) electrode modified by TiO2 inverse opal (TiO2(IO)). Electrochemical properties of GOx/TiO2(IO)/ITO electrode were characterized by using the three electrodes system. The result of cyclic voltammetry showed that a couple of stable and well-defined redox peaks for the direct electron transfer of GOx in absence of glucose, and the redox peak height enhanced in presence of 0.1 mu M glucose. Compare with the ordinary structured GOx/TiO2/ITO electrode, inverse opal structured GOx/TiO2(IO)/ITO electrode has a better respond to the glucose concentration change. Under optimized experimental conditions of solution pH 6.8 and detection potential at 0.30 V versus saturated calomel electrode (SCE), amperometric measurements were performed. The sensitivity and the detection limit of glucose detection was 151 mu A cm(-2) mM(-1) and 0.02 mu M at a signal-to-noise ratio of 3, respectively. The good response was due to the good biocompatibility of TiO2 and the large effective surface of the three-dimensionally ordered macroporous structure.
机译：3D大孔TiO2反蛋白石是使用聚苯乙烯胶体晶体作为模板的溶胶-凝胶法制得的。 EDS和SEM表明获得了面心立方（FCC）结构的TiO2反蛋白石。葡萄糖氧化酶（GOx）成功地固定在TiO2反蛋白石（TiO2（IO））修饰的铟锡氧化物（ITO）电极的表面上。采用三电极体系表征了GOx / TiO2（IO）/ ITO电极的电化学性能。循环伏安法的结果表明，在葡萄糖不存在的情况下，GOx的直接电子转移有两个稳定且定义明确的氧化还原峰，而在0.1μM葡萄糖存在下，氧化还原峰高度增加。与普通结构的GOx / TiO2 / ITO电极相比，反蛋白石结构的GOx / TiO2（IO）/ ITO电极对葡萄糖浓度的变化具有更好的响应。在优化的溶液pH 6.8的实验条件下以及在相对于饱和甘汞电极（SCE）的0.30 V下的检测电势下，进行了安培测量。葡萄糖检测的灵敏度和检测极限分别为151μA cm（-2）mM（-1）和0.02μM，信噪比为3。良好的响应归因于TiO2的良好生物相容性和三维有序大孔结构的有效表面大。

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