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首页> 外文期刊>Electroanalysis >Electrocatalysis via direct electrochemistry of myoglobin immobilized on colloidal gold nanoparticles
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Electrocatalysis via direct electrochemistry of myoglobin immobilized on colloidal gold nanoparticles

机译：通过直接电化学法对固定在胶体金纳米颗粒上的肌红蛋白进行电催化

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The direct electron transfer between immobilized myoglobin (Mb) and colloidal gold modified carbon paste electrode was studied. The Mb immobilized on the colloidal gold nanoparticles displayed a pair of redox peaks in 0.1 M pH 7.0 PBS with a formal potential of -(0.108 +/- 0.002) V (vs. NHE). The response showed a surface -controlled electrode process with an electron transfer rate constant of (26.7 +/- 3.7) s(-1) at scan rates from 10 to 100 mV s(-1) and a diffusioncontrolled process involving the diffusion of proton at scan rates more than 100 mV s(-1). The immobilized Mb maintained its activity and could electrocatalyze the reduction of both hydrogen peroxide and nitrite. Thus, the novel renewable reagentless sensors for hydrogen peroxide and nitrite were developed, respectively. The activity of Mb with respect to the pseudo peroxidase with a K (app)(M) value of 0.65 mM could respond linearly to hydrogen peroxide concentration from 4.6 to 28 muM. ne sensor exhibited a fast amperometric response to NO2- reduction and reached 93% of steady-state current within 5 s. The linear range for NO2- determination was from 8.0 to 112 muM with a detection limit of 0.7 M at 3a. [References: 44]
机译：研究了固定化肌红蛋白（Mb）与胶体金修饰碳糊电极之间的直接电子转移。固定在胶体金纳米颗粒上的Mb在0.1 M pH 7.0 PBS中显示一对氧化还原峰，其形式势为-（0.108 +/- 0.002）V（相对NHE）。该响应显示了一个表面控制的电极过程，在10到100 mV s（-1）的扫描速率下，电子传递速率常数为（26.7 +/- 3.7）s（-1），并且涉及质子扩散的扩散控制过程扫描速率超过100 mV s（-1）。固定化的Mb保持其活性，并且可以电催化过氧化氢和亚硝酸盐的还原。因此，分别开发了用于过氧化氢和亚硝酸盐的新型可再生无试剂传感器。 Mb相对于K（app）（M）值为0.65 mM的假过氧化物酶的活性可以线性反应4.6至28μM的过氧化氢浓度。 ne传感器对NO2-还原具有快速的安培响应，并在5 s内达到稳态电流的93％。 NO2-测定的线性范围是8.0至112μM，在3a处的检测极限为0.7M。 [参考：44]

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