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首页> 外文期刊>Electroanalysis >Electrooxidation of DNA at glassy carbon electrodes modified with multiwall carbon nanotubes dispersed in chitosan
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Electrooxidation of DNA at glassy carbon electrodes modified with multiwall carbon nanotubes dispersed in chitosan

机译：壳聚糖中分散的多壁碳纳米管修饰的玻璃碳电极上DNA的电氧化

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摘要

We report on the analytical performance of glassy carbon (GCE) electrodes modified with a dispersion of multiwall carbon nanotubes (CNT) in chitosan (CHIT) for the quantification of DNA. The electroanalytical response of the resulting electrodes was evaluated using differential pulse voltammetry, while the electrochemical reactivity of the film surface was characterized using scanning electrochemical microscopy. Different treatments of the modified GCE were evaluated to improve the stability of the film and the accumulation of DNA. The guanine oxidation signal of double stranded calf-thymus DNA after 3-min accumulation was 20 times higher at GCE/CHIT-CNT cross-linked with glutaraldehyde (GTA) than at bare GCE, while the peak potential was around 45 mV less positive. The guanine oxidation signal demonstrated to be highly reproducible, with 3.4% RSD for 5 different electrodes. The treatment with sodium hydroxide demonstrated to be not effective since the resulting films were less stable and the guanine oxidation signal was ten times smaller compared to electrodes prepared with the GTA treated films. The effect of chitosan molecular weight used to prepare the dispersion and the amount of carbon nanotubes dispersed were evaluated. The response of single stranded DNA and oligo(dG)(15) is also discussed.
机译：我们报告了修饰的玻璃碳（GCE）电极在壳聚糖（CHIT）中的多壁碳纳米管（CNT）分散体改性的定量DNA的分析性能。使用差分脉冲伏安法评估所得电极的电分析响应，同时使用扫描电化学显微镜表征膜表面的电化学反应性。评估了改性GCE的不同处理方法，以改善薄膜的稳定性和DNA的积累。与戊二醛（GTA）交联的GCE / CHIT-CNT的3分钟累积后，双链小牛-胸腺DNA的鸟嘌呤氧化信号比裸GCE时高20倍，而峰值电位则低45 mV。鸟嘌呤氧化信号显示出高度可重复性，对5个不同的电极具有3.4％的RSD。用氢氧化钠处理证明是无效的，因为与用GTA处理的膜制备的电极相比，所得膜的稳定性较差并且鸟嘌呤氧化信号小十倍。评价用于制备分散体的壳聚糖分子量的影响和分散的碳纳米管的量。还讨论了单链DNA和oligo（dG）（15）的响应。

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