...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Electroanalysis >Effect of Basic Amino Acids on Nickel Ion Reduction at a Mercury Electrode
【24h】

Effect of Basic Amino Acids on Nickel Ion Reduction at a Mercury Electrode

机译：碱性氨基酸对汞电极上镍离子还原的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In a 0.02 M borax solution (pH 8.5), basic amino acids (arginine, lysine, and ornithine) react with Nit+ to form a mono-ligand complex that is reduced at a mercury electrode at about _ 0.85 V vs. Ag l AgCl I KC1 (3 M). At a long time scale (staircase voltammetry; scan rate < 50 mV s~(-1)), the complex reduction is a catalytic (EC') process, the rate-determining step being the regeneration of the reducible species by the reaction of the amino acid with free Ni~(2+). At a short time scale (differential pulse voltammetry or higher scan rate staircase voltammetry), the reaction rate is controlled by the diffusion of the complex. Although the same kind of complexation occurs with either basic amino acids or glycine, the last one does not induce a similar process. The peculiar effect of basic amino acids is due to the side chain that causes the ligand molecule to adopt a favorable orientation at the electrode surface. The differential pulse voltammetry peak current is proportional to the total amino acid concentration over the concentration range from 2 to 100 μM. Hence a voltammetric method for arginine determination in nutritional supplements was developed and validated using HPLC as reference method.
机译：在0.02 M硼砂溶液（pH 8.5）中，碱性氨基酸（精氨酸，赖氨酸和鸟氨酸）与Nit +反应形成单配体络合物，该汞在汞电极上相对于Ag l AgCl I在约_ 0.85 V时还原KC1（3 M）。在长时间范围内（楼梯伏安法;扫描速率<50 mV s〜（-1）），络合物的还原是催化（EC'）过程，速率确定步骤是通过以下反应进行可还原物质的再生带有游离Ni〜（2+）的氨基酸。在较短的时间范围内（差分脉冲伏安法或较高扫描速率阶梯伏安法），反应速率由配合物的扩散控制。尽管碱性氨基酸或甘氨酸都发生相同类型的络合，但最后一种不会诱导相似的过程。碱性氨基酸的特殊作用是由于侧链导致配体分子在电极表面采取有利的取向。在2至100μM的浓度范围内，差分脉冲伏安法峰值电流与总氨基酸浓度成正比。因此，开发了伏安法测定营养补品中的精氨酸，并使用HPLC作为参考方法进行了验证。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号