...
首页> 外文期刊>Electroanalysis >Electrochemical magnetic immunosensors for the determination of ceruloplasmin
【24h】

Electrochemical magnetic immunosensors for the determination of ceruloplasmin

机译:电化学磁免疫传感器测定铜蓝蛋白

获取原文
获取原文并翻译 | 示例
           

摘要

Electrochemical immunosensors for ceruloplasmin (Cp) are reported for the first time. Two configurations involving magnetic beads (MBs) functionalized with Protein A or Streptavidin for immobilization of Cp antibodies were compared, using competitive immunoassay with synthesized alkaline phosphatase-Cp conjugate. Upon capturing MBs-immunoconjugates onto screen-printed carbon electrodes, quantification of Cp was accomplished by DPV measurement of 1-naphthol generated after 1-naphthylphosphate addition. Linear ranges of calibration curves and detection limits were 0.1-1000μg/mL and 0.040μg/mL (Protein A-MBs), and 0.025-20μg/mL and 0.018μg/mL (Strept-MBs). Good results were obtained in the determination of Cp in spiked human serum samples.
机译:铜蓝蛋白(Cp)的电化学免疫传感器是第一次报道。使用具有合成的碱性磷酸酶-Cp共轭物的竞争性免疫测定法,比较了两种涉及用蛋白A或链霉亲和素功能化的磁珠(MBs)固定Cp抗体的构型。在将MBs-免疫缀合物捕获到丝网印刷的碳电极上后,通过DPV测量添加1-萘基磷酸后生成的1-萘酚来完成Cp的定量。校正曲线和检出限的线性范围为0.1-1000μg/ mL和0.040μg/ mL(蛋白质A-MBs),以及0.025-20μg/ mL和0.018μg/ mL(Strept-MBs)。测定加标的人血清样品中的Cp可获得良好的结果。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号