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首页> 外文期刊>Electroanalysis >Multiwalled Carbon Nanotube-CaCO_3 Nanoparticle Composites for the Construction of a Tyrosinase-Based Amperometric Dopamine Biosensor
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Multiwalled Carbon Nanotube-CaCO_3 Nanoparticle Composites for the Construction of a Tyrosinase-Based Amperometric Dopamine Biosensor

机译：多壁碳纳米管-CaCO_3纳米粒子复合材料用于构建基于酪氨酸酶的安培多巴胺生物传感器。

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摘要

We report the fabrication of a highly sensitive dopamine biosensor based on the entrapment of tyrosinase into CaCO_3 nanoparticles at Multiwalled Carbon Nanotube (MWCNT) electrodes. CaCO_3 acts as host matrix for tyrosinase and MWCNT provides a highly porous conductive network enhancing the enzyme immobilization and the electrochemical transduction of the enzyme reaction by boosting the amplification phenomenon involved in the biosensing of catechol and dopamine. The comparison of the performance of CaCO_3-tyrosinase electrodes with and without MWCNT film clearly indicates the improvement in sensitivity and maximum current brought by the combination of MWCNTs and inorganic nanomaterials. These nanostructured hybrid bioelectrodes exhibit a high sensitivity for the detection of catechol and dopamine, namely 35.7Amol~(-1)Lcm~(-2), the detection limit for dopamine being 15nmolL~(-1) with no influence of the presence of interferents, i.e. uric acid and ascorbic acid.
机译：我们报告了基于酪氨酸酶在多壁碳纳米管（MWCNT）电极上将酪氨酸酶截留到CaCO_3纳米颗粒中的制造。 CaCO_3充当酪氨酸酶的宿主基质，MWCNT提供了高度多孔的导电网络，可通过促进涉及儿茶酚和多巴胺生物传感的扩增现象来增强酶的固定化和酶反应的电化学转导。有和没有MWCNT膜的CaCO_3-酪氨酸酶电极性能的比较清楚地表明，MWCNT和无机纳米材料的结合带来了灵敏度和最大电流的提高。这些纳米结构的杂化生物电极对儿茶酚和多巴胺的检测具有很高的灵敏度，即35.7Amol〜（-1）Lcm〜（-2），多巴胺的检测限为15nmolL〜（-1），而没有检测到多巴胺。干扰物，即尿酸和抗坏血酸。

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