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首页> 外文期刊>Electroanalysis >Voltammetric behavior of osmium-labeled DNA at mercury meniscus-modified solid amalgam electrodes. Detecting DNA hybridization
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Voltammetric behavior of osmium-labeled DNA at mercury meniscus-modified solid amalgam electrodes. Detecting DNA hybridization

机译：mercury标记的DNA在汞弯月面修饰的固态汞合金电极上的伏安行为。检测DNA杂交

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摘要

The complex of osmium tetroxide with 2,2'-bipyridine has been utilized as a probe of DNA structure and all electroactive marker of DNA in DNA hybridization sensors. It produces several voltammetric signals, the most negative of them has been observed only at mercury electrodes. This signal is of catalytic nature affording a high sensitivity of DNA determination. The catalytic current due to evolution of hydrogen in voltammetry of DNA modified by complex of osmium tetroxide with 2,2'-bipyridine (DNA-Os,bipy) was studied. Solid amalgam electrodes (modified with mercury menisci) of silver (m-AgSAE), copper (m-CuSAE), gold. and of combined bismuth and silver, Were used as possible Substitutes for mercury electrodes. Besides the hanging mercury drop electrode (HMDE), the catalytic Current was observed only on m-AgSAE and m-CuSAE. Electrodes of gold and bismuth amalgams did not give the catalytic Current. The detection limit of DNA-Os,bipy on HMDE was 0.1 ng mL(-1) (RSD = 2.3% N = 11) and oil m-AgSAE 0.2 ng mL(-1) (RSD = 3.1.%, N = 11). The m-AgSAE was successfully applied as a detection electrode in double-surface DNA hybridization experiments offering highly specific discrimination between complementary (target) and nonspecific DNAs, as well as determination of the length of a repetitive DNA sequence. The m-AgSAE has proved a convenient alternative to the HMDE or carbon electrodes used for similar purposes in previous work.
机译：四氧化与2,2'-联吡啶的复合物已被用作DNA杂交传感器中DNA结构的探针和DNA的所有电活性标记。它产生几个伏安信号，其中最负的信号仅在汞电极上观察到。此信号具有催化性质，可提供高度的DNA测定灵敏度。研究了四氧化与2,2'-联吡啶（DNA-Os，bipy）配合物修饰的DNA的伏安法中氢的析出电流。固态汞齐电极（用汞弯月面修饰）包括银（m-AgSAE），铜（m-CuSAE），金。以及铋和银的混合物，被用作汞电极的替代品。除了悬挂式汞滴电极（HMDE），仅在m-AgSAE和m-CuSAE上观察到催化电流。金和铋汞合金的电极未产生催化电流。 HMDE上的DNA-Os，bipy的检出限为0.1 ng mL（-1）（RSD = 2.3％N = 11）和m-AgSAE油为0.2 ng mL（-1）（RSD = 3.1。％，N = 11） ）。 m-AgSAE已成功地用作双面DNA杂交实验中的检测电极，可提供互补（靶标）和非特异性DNA的高度特异性区分，以及确定重复DNA序列的长度。事实证明，m-AgSAE可以替代先前工作中用于类似目的的HMDE或碳电极。

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