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PAMAM dendrimers-based DNA biosensors for electrochemical detection of DNA hybridization

机译：基于PAMAM树状聚合物的DNA生物传感器用于DNA杂交的电化学检测

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摘要

Gold electrodes were modified with submonolayers of mercaptoacetic acid (RSH) and further reacted with poly(amidoamine) (PAMAM) dendrimers (generation 4.0) to obtain thin films, on which DNA probe was later immobilized to afford a stable recognition layers. The characterization of the PAMAM/RSH-modified electrode was investigated by cyclic voltammetry (CV) and electrochemical impedance measurement. Differential pulse voltammogram (DPV) measurement was used to monitor DNA hybridization with daunomycin (DNR) as indicator. Experiments carried out with these novel materials not only showed an improved DNA attachment quantity on the dendrimers-modified electrodes compared to DNA sensors with oligonucleotides directly immobilized on Au electrodes, but also exhibited a high selectivity, sensitivity and stability for the measurement of DNA hybridization.
机译：用巯基乙酸（RSH）的亚单层修饰金电极，然后使其与聚（酰胺基胺）（PAMAM）树状大分子（4.0代）反应以获得薄膜，随后将其固定在DNA探针上以提供稳定的识别层。通过循环伏安法（CV）和电化学阻抗测量研究了PAMAM / RSH修饰电极的特性。差示脉冲伏安法（DPV）测量用于监测以道诺霉素（DNR）为指示剂的DNA杂交。用这些新颖的材料进行的实验不仅表明，与直接固定在Au电极上的寡核苷酸的DNA传感器相比，树状聚合物修饰的电极上的DNA附着量得到了改善，而且对DNA杂交的测量显示出很高的选择性，灵敏度和稳定性。

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