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首页> 外文期刊>Electroanalysis >Continuous monitoring of L-glutamate released from cultured rat nerve cells with a microfabricated on-line sensor at a slow flow rate
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Continuous monitoring of L-glutamate released from cultured rat nerve cells with a microfabricated on-line sensor at a slow flow rate

机译：用微细的在线传感器以缓慢的流速连续监测从培养的大鼠神经细胞释放的L-谷氨酸

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A microfabricated online L-glutamate sensor was developed to monitor changes in the local concentration of L-glutamate released from cultured nerve cells. The sensor consists of two glass plates and a fused silica sampling capillary. One of the glass plates has rectanglar flow channels cut into it with a dicing saw and the other has three carbon film based electrodes. One of the electrodes was modified with an Os-polyvinylpyrridine-based polymer layer containing horseradish peroxidase. A GluOx enzyme layer was immobilized upstream of the working electrode. A syringe pump in the suction mode was used to sample extracellular fluid continuously via a glass capillary with a pointed end. The sensor sensitivity did not decrease with decreasing flow rate when the Bow rate was reduced to about 200 nL/min. This is because the increase in the conversion efficiency compensated for the decrease in the analyte flow into the sensor. As a result, a low detection limit (6.4 nM) was obtained at a slow flow rate. In an in vitro experiment, the extracellular fluid near a particular nerve cell was sampled through a capillary and continuously introduced into the sensor via the suction provided by a syringe pump. The nerve cells were stimulated by KCl and a transient L-glutamate release with a sharp peak was monitored with a slow Bow rate of 500 nL/min to 2 mu L/min. [References: 35]
机译：开发了一种微型的在线L-谷氨酸传感器，以监测从培养的神经细胞释放的L-谷氨酸的局部浓度的变化。该传感器由两个玻璃板和一个熔融石英采样毛细管组成。其中一块玻璃板具有用划片机切入的矩形流动通道，另一块具有三个基于碳膜的电极。用包含辣根过氧化物酶的基于Os-聚乙烯基吡啶的聚合物层修饰电极之一。 GluOx酶层固定在工作电极的上游。抽吸模式下的注射泵用于通过带尖端的玻璃毛细管连续采样细胞外液。当Bow速率降低到约200 nL / min时，传感器的灵敏度不会随着流速的降低而降低。这是因为转换效率的提高补偿了流入传感器的分析物流量的减少。结果，在低流速下获得了低检测限（6.4nM）。在体外实验中，通过毛细管对特定神经细胞附近的细胞外液进行采样，并通过注射泵提供的吸力将其连续引入传感器中。用KCl刺激神经细胞，并以500 nL / min至2μL / min的缓慢Bow速率监测具有尖峰的L-谷氨酸的瞬时释放。 [参考：35]

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