A Genosensor for Point Mutation Detection of P53 Gene PCR Product Using Magnetic Particles

Hamidi-Asl Ezat; Raoof Jahan Bakhsh; Hejazi Mohammad Saeid; Sharifi Simin; Golabi Seyed Mahdi; Palchetti Ilaria; Mascini Marco

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A Genosensor for Point Mutation Detection of P53 Gene PCR Product Using Magnetic Particles

机译：一种利用磁性粒子检测P53基因PCR产物点突变的基因传感器

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The development of an electrochemical genosensor involving DNA biotinylated capture probe immobilized on streptavidin coated paramagnetic beads and microfluidic based platform for the detection of P53 gene PCR product is reported. The novelty of this work is the combination of a sensitive electrochemical platform and a proper microfluidic system with a simple and effective enzyme signal amplification technology, ELISA, for detection of target DNA sequence and single nucleotide mutation in p53 tumor suppressor gene sequence. The biosensor has been applied to detect the PCR amplified samples and the results shows that it can discriminate successfully perfect matched DNA from mutant form.

机译：据报道，电化学基因传感器的开发涉及固定在链霉抗生物素蛋白包被的顺磁珠上的DNA生物素化捕获探针和用于检测P53基因PCR产物的基于微流体的平台。这项工作的新颖之处在于将灵敏的电化学平台和适当的微流体系统与简单有效的酶信号放大技术ELISA相结合，可检测目标DNA序列和p53抑癌基因序列中的单核苷酸突变。该生物传感器已用于检测PCR扩增的样品，结果表明它可以成功地将完美匹配的DNA与突变体形式区分开。

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来源
《Electroanalysis》 |2015年第6期|共9页
作者
Hamidi-Asl Ezat; Raoof Jahan Bakhsh; Hejazi Mohammad Saeid; Sharifi Simin; Golabi Seyed Mahdi; Palchetti Ilaria; Mascini Marco;
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正文语种 eng
中图分类电化学、电解、磁化学;
关键词
Magnetic beads; ELISA; Sandwich hybridization; Microfluidic platform; PCR products;

机译：磁珠ELISA夹心杂交微流控平台PCR产物;

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专利

1. A Genosensor for Point Mutation Detection of P53 Gene PCR Product Using Magnetic Particles [J] . Hamidi-Asl Ezat, Raoof Jahan Bakhsh, Hejazi Mohammad Saeid, Electroanalysis . 2015,第6期

机译：一种利用磁性粒子检测P53基因PCR产物点突变的基因传感器
2. Detection of p53 gene mutations in human leukemia by PCR-SSCP analysis and direct nucleotide sequencing [J] . Wang Rong, Yu Zhifei, Gu Jianren Chinese Medical Journal . 1998,第4期

机译：通过PCR-SSCP分析和直接核苷酸测序检测人白血病中的p53基因突变
3. Sensitive detection of p53 gene mutations by a ‘mutant enriched' PCR-SSCP technique [J] . Marcus Schuermann, Moira Behn Nucleic acids research . 1998,第5期

机译：通过“突变富集” PCR-SSCP技术灵敏检测p53基因突变
4. Introduction of an Electrochemical Genosensor for Detection of P53 Gene Via Sandwich Hybridization Method [C] . Ezat Hamidi-Asl, Ilaria Palchetti, Marco Mascini Sensors and microsystems . 2011

机译：夹心杂交法检测P53基因的电化学基因传感器的介绍
5. Design, Construction, and Verification of a LATE-PCR and Lights-On/Lights-Off Probe Assay for Mutation Detection in Exons 18-21 of the EGFR Gene: Implications for Molecular Diagnosis of Non-Small Cell Lung Carcinoma [D] . Tetrault, Shana Augustin 2012

机译：用于EGFR基因外显子18-21突变检测的LATE-PCR和开/关灯探针检测的设计，构建和验证：对非小细胞肺癌的分子诊断意义
6. A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au [O] . Mohadeseh Zarei-Ghobadi, Sayed-Hamidreza Mozhgani, Fariba Dashtestani, -1

机译：一种在铁磁性纳米粒子封端的Au存在下基于荧光碳点的光致发光猝灭来检测HTLV-I的基因传感器
7. Simultaneous detection of homozygous deletion and point mutations in tumor suppressor genes p16 and p53 by multiplex-PCR/SSCP analysis [O] . Papp Thilo, Pemsel Heidi, Weiss Dieter G., 1997

机译：通过多重PCR / SSCP分析同时检测肿瘤抑制基因p16和p53中的纯合缺失和点突变

A Genosensor for Point Mutation Detection of P53 Gene PCR Product Using Magnetic Particles

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